Sex steroids in the female zebrafish (Danio rerio). Effects of cyproterone acetate and leachate-contaminated sediment extract
2006 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 79, no 2, 192-200 p.Article in journal (Refereed) Published
In Molnbyggen, a leachate-contaminated lake in Sweden, effects on the reproductive system of perch included a decreased frequency of sexually mature female perch, reduced gonadosomatic index and decreased plasma levels of androstenedione and testosterone, but the contaminants responsible for these effects have not yet been identified. A biomarker-directed fractionation approach could be used to narrow the search for the compound(s) responsible for the adverse effects in Molnbyggen female perch. Thus, it is important to develop and test a suitable biomarker, which can be used in this type of approach. We evaluated if decreased concentrations of sex steroids could work as an in vivo end-point in female zebrafish fed an organic extract of bottom sediments from Molnbyggen. The responsiveness of zebrafish to endocrine alteration was evaluated using a positive control substance, cyproterone acetate (CA), which is known to inhibit steroid biosynthesis. After the 21-day exposure period the concentrations of testosterone (T) and estradiol (E2) were successfully determined by HRGC/HRMS in whole-body homogenates of individual fish. Median T concentrations were 1.7 pmol/g fish in controls and were decreased after exposure both to CA (0.55 pmol/g fish) and Molnbyggen sediment extract (1.2–1.3 pmol/g fish). Median E2 concentrations were 5.3 pmol/g fish in controls and were decreased after CA exposure (2.3 pmol/g fish) and after exposure to Molnbyggen sediment extract (4.0–4.5 pmol/g fish). This suggests that sex steroid concentrations in zebrafish are responsive to endocrine alteration and indicates that compounds with endocrine altering potency are present in Molnbyggen sediments.
Place, publisher, year, edition, pages
Elsevier B.V , 2006. Vol. 79, no 2, 192-200 p.
Zebrafish; Sex steroids; Cyproterone acetate; Leachate; Sediment; Endocrine disruption
IdentifiersURN: urn:nbn:se:su:diva-25870DOI: 10.1016/j.aquatox.2006.06.011OAI: oai:DiVA.org:su-25870DiVA: diva2:200711
Part of urn:nbn:se:su:diva-9512006-04-122006-04-122010-10-04Bibliographically approved