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Sex steroids in the female zebrafish (Danio rerio). Effects of cyproterone acetate and leachate-contaminated sediment extract
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK), Environmental Chemistry.
Stockholm University, Faculty of Science, Department of Materials and Environmental Chemistry (MMK), Environmental Chemistry.
2006 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 79, no 2, 192-200 p.Article in journal (Refereed) Published
Abstract [en]

In Molnbyggen, a leachate-contaminated lake in Sweden, effects on the reproductive system of perch included a decreased frequency of sexually mature female perch, reduced gonadosomatic index and decreased plasma levels of androstenedione and testosterone, but the contaminants responsible for these effects have not yet been identified. A biomarker-directed fractionation approach could be used to narrow the search for the compound(s) responsible for the adverse effects in Molnbyggen female perch. Thus, it is important to develop and test a suitable biomarker, which can be used in this type of approach. We evaluated if decreased concentrations of sex steroids could work as an in vivo end-point in female zebrafish fed an organic extract of bottom sediments from Molnbyggen. The responsiveness of zebrafish to endocrine alteration was evaluated using a positive control substance, cyproterone acetate (CA), which is known to inhibit steroid biosynthesis. After the 21-day exposure period the concentrations of testosterone (T) and estradiol (E2) were successfully determined by HRGC/HRMS in whole-body homogenates of individual fish. Median T concentrations were 1.7 pmol/g fish in controls and were decreased after exposure both to CA (0.55 pmol/g fish) and Molnbyggen sediment extract (1.2–1.3 pmol/g fish). Median E2 concentrations were 5.3 pmol/g fish in controls and were decreased after CA exposure (2.3 pmol/g fish) and after exposure to Molnbyggen sediment extract (4.0–4.5 pmol/g fish). This suggests that sex steroid concentrations in zebrafish are responsive to endocrine alteration and indicates that compounds with endocrine altering potency are present in Molnbyggen sediments.

Place, publisher, year, edition, pages
Elsevier B.V , 2006. Vol. 79, no 2, 192-200 p.
Keyword [en]
Zebrafish; Sex steroids; Cyproterone acetate; Leachate; Sediment; Endocrine disruption
National Category
Environmental Sciences
Identifiers
URN: urn:nbn:se:su:diva-25870DOI: 10.1016/j.aquatox.2006.06.011OAI: oai:DiVA.org:su-25870DiVA: diva2:200711
Note
Part of urn:nbn:se:su:diva-951Available from: 2006-04-12 Created: 2006-04-12 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Biochemical characterisation of landfill leachate toxicity in fish
Open this publication in new window or tab >>Biochemical characterisation of landfill leachate toxicity in fish
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Deposition remains the most common form of waste disposal in many parts of the world. As a terminus of the products consumed in our society a landfill may contain virtually all sorts of man-made chemicals. Despite this, the harmfulness of landfill leachate has not been extensively evaluated in feral organisms in the environment. In a leachate-contaminated lake, Molnbyggen, in Sweden, our studies reported a low percent of sexually mature (SM) female perch (Perca fluviatilis) that had decreased plasma androgen levels, decreased brain aromatase activity, distinctive sores and fin erosion. The impairments were attributed to unidentified compound(s) present in the leachate. In one out of four other investigated leachate-contaminated lakes, the low percent of SM female perch had reduced plasma sex steroid levels and similar sores as perch in Molnbyggen. The biochemical mechanism causing the disorders was investigated in order to establish a connection between the impairments and possible causative compound(s). Plasma levels of progesterone and 17α-hydroxyprogesterone were unaffected. Ovarian 17,20-lyase activity was decreased while levels of biliary steroid conjugates and hepatic testosterone UDP-glucuronosyltransferase activity did not differ between exposed and reference SM fish. Furthermore, the decreased brain aromatase activity seems to be a secondary effect; possibly a result of low substrate availability. Altogether, this suggests a possible disruption in the synthesis of androgens, knowledge that could be used as a tool in biomarker-directed fractionation studies to pinpoint compound(s). Molnbyggen sediment extracts decreased the testosterone and estradiol concentrations in whole-body homogenates of zebrafish (Danio rerio) after a three week exposure period. This suggests that compound(s) with the potency to alter endocrine function are present in the sediment. Although the first steps have been taken towards identifying compound(s) responsible for this kind of reproductive impairments, they still remain unidentified. Measures have to be taken to identify harmful chemicals in our society, to reduce their number, and to minimise their uncontrolled dispersal.

Place, publisher, year, edition, pages
Stockholm: Institutionen för tillämpad miljövetenskap (ITM), 2006. 44 p.
Keyword
landfill leachate, endocrine disruption, fish
National Category
Environmental Sciences
Identifiers
urn:nbn:se:su:diva-951 (URN)91-7155-232-4 (ISBN)
Public defence
2006-05-12, Ahlmansalen, Geovetenskapens hus, Svante Arrhenius väg 8, Stockholm, 10:00
Opponent
Supervisors
Available from: 2006-04-12 Created: 2006-04-12Bibliographically approved

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