Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Physiological regulation of the high-affinity phosphate uptake system in the yeast Saccharomyces cerevisiae
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Yeast cells can be found in a wide variety of environments, demonstrating that these cells are capable of adapting to changes in the external milieu. Inorganic phosphate (Pi) is an essential nutrient for all organisms and Saccharomyces cerevisiae cells take up phosphate from the external environment via two different systems, i.e., a high-affinity system consisting of the Pho84 and Pho89 permeases and a low-affinity system composed of the Pho87, Pho90 and Pho91 permeases. Expression of the Pho84 and Pho89 permeases is regulated in response to the extracellular concentration of Pi, whereas the low-affinity system is thought to be expressed constitutively. The Pho84 permease couples phosphate uptake with protons and has an acidic pH optimum. In contrast, Pho89 is a cation-coupled phosphate transporter with a preference for sodium ions and an alkaline pH optimum.

In the present thesis the regulation of the two high-affinity phosphate transporters in response to alterations in the external concentration of Pi has been examined. The Pho84 permease is the major supplier of phosphate under Pi-limiting conditions, whereas the Pho89 permease plays only a minor role under such conditions. Instead, Pho89 is proposed to be involved in supplying phosphate to the cells in different stressful situations. In a strain from which all of the phosphate transporters except Pho89 have been deleted, this protein is up-regulated sufficiently to sustain cell growth under both high- and low-phosphate conditions even in the presence of an acidic environment.

In addition to being regulated on the transcriptional level, Pho84 is also regulated post-translationally and is in response to Pi addition or depletion internalised and sorted to the vacuole for degradation. This post-translational regulation is independent of the PHO pathway that mediates transcriptional regulation.

Place, publisher, year, edition, pages
Stockholm: Institutionen för biokemi och biofysik , 2006. , 42 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-988ISBN: 91-7155-235-9 (print)OAI: oai:DiVA.org:su-988DiVA: diva2:200792
Public defence
2006-05-22, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, Stockholm, 14:00
Opponent
Supervisors
Available from: 2006-04-28 Created: 2006-04-28Bibliographically approved
List of papers
1. Intracellular localization of an active green fluorescent protein-tagged Pho84 phosphate permease in Saccharomyces cerevisiae.
Open this publication in new window or tab >>Intracellular localization of an active green fluorescent protein-tagged Pho84 phosphate permease in Saccharomyces cerevisiae.
Show others...
1999 In: FEBS Letters, ISSN 0014-5793, Vol. 462, no 1-2, 37-42 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-25909 (URN)
Note
Part of urn:nbn:se:su:diva-988Available from: 2006-04-28 Created: 2006-04-28Bibliographically approved
2. Regulation of cation-coupled high-affinity phosphate uptake in the yeast Saccharomyces cerevisiae.
Open this publication in new window or tab >>Regulation of cation-coupled high-affinity phosphate uptake in the yeast Saccharomyces cerevisiae.
2000 In: Journal of bacteriology, ISSN 0021-9193, Vol. 182, no 17, 5017-5019 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-25910 (URN)
Note
Part of urn:nbn:se:su:diva-988Available from: 2006-04-28 Created: 2006-04-28Bibliographically approved
3. Mutagenic and functional analysis of the C-terminus of Saccharomyces cerevisiae Pho84 phosphate transporter.
Open this publication in new window or tab >>Mutagenic and functional analysis of the C-terminus of Saccharomyces cerevisiae Pho84 phosphate transporter.
Show others...
2002 In: FEBS Letters, ISSN 0014-5793, Vol. 526, no 1-3, 31-37 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-25911 (URN)
Note
Part of urn:nbn:se:su:diva-988Available from: 2006-04-28 Created: 2006-04-28Bibliographically approved
4. Effects of methylphosphonate, a phosphate analogue, on the expression and degradation of the high-affinity phosphate transporter Pho84, in Saccharomyces cerevisiae.
Open this publication in new window or tab >>Effects of methylphosphonate, a phosphate analogue, on the expression and degradation of the high-affinity phosphate transporter Pho84, in Saccharomyces cerevisiae.
Show others...
2004 In: Biochemistry, ISSN 0006-2960, Vol. 43, no 45, 14444-14453 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-25912 (URN)
Note
Part of urn:nbn:se:su:diva-988Available from: 2006-04-28 Created: 2006-04-28Bibliographically approved
5. The role of Pho89, a cation-coupled high-affinity phosphate transporter from Saccharomyces cerevisiae, as a rescue system.
Open this publication in new window or tab >>The role of Pho89, a cation-coupled high-affinity phosphate transporter from Saccharomyces cerevisiae, as a rescue system.
Show others...
Manuscript (Other academic)
Identifiers
urn:nbn:se:su:diva-25913 (URN)
Note
Part of urn:nbn:se:su:diva-988Available from: 2006-04-28 Created: 2006-04-28 Last updated: 2010-01-13Bibliographically approved

Open Access in DiVA

No full text

By organisation
Department of Biochemistry and Biophysics
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 159 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf