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Peroxisomal proteomics, biomonitoring in mussles after the Prestige's oil spill
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Department of Zoology and Cell Biology, University of the Basque Country.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
2009 (English)In: Marine Pollution Bulletin, ISSN 0025-326X, E-ISSN 1879-3363, Vol. 58, no 12, 1815-1826 p.Article in journal (Refereed) Published
Abstract [en]

Peroxisomal proteomics was applied to assess possible biological effects after the Prestige's oil spill. Mussels were sampled in July 2004 and 2005 in four stations in the NW (closest to the spill) and NE coasts of the Iberian Peninsula. Principal components analysis (PCA) suggested differences in protein expression among stations and sampling years. Several proteins were putatively identified by mass spectrometry and immunolocalization. PC1 separated the NW stations in 2004 from the rest of the stations and sampling years mainly due to up-regulation of peroxisomal β-oxidation proteins and PMP70. PC3 separated the NE-stations, based on up-regulation of the antioxidant enzyme catalase in 2004 compared to 2005. PC4 separated the stations in the NE and the NW. This work shows that environmental proteomics, together with multivariate data analysis, could provide information to interpret the effects of oil spills at cellular level in mussels also in the absence of historical data.

Place, publisher, year, edition, pages
2009. Vol. 58, no 12, 1815-1826 p.
Keyword [en]
proteomics, biomarker, two-dimensional electrophoresis, marine pollution assessment, oil spill, Prestige
URN: urn:nbn:se:su:diva-26585DOI: 10.1016/j.marpolbul.2009.08.004ISI: 000273104200018OAI: diva2:210510
Available from: 2009-04-02 Created: 2009-04-02 Last updated: 2010-08-11Bibliographically approved
In thesis
1. Development and application of a proteomic approach to the assessment of pollution in the marine environment
Open this publication in new window or tab >>Development and application of a proteomic approach to the assessment of pollution in the marine environment
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Today, assessment of the health of coastal waters is recognized as being important for both the conservation of nature and well-being of humans. Anthropogenic pollution has been the focus of extensive research for some time and a variety of programs for the monitoring and assessment of environmental pollution have been developed. Determination of the levels of pollution in sensitive ‘sentinels’ such as mussels, allows monitoring of these levels in a given area over a prolonged period of time. Furthermore, the biological effects of pollution are reflected in a series of biomarkers, none of which provides a general picture of the sentinel’s state of health and all of which are individually specific for certain pollutants and influenced by both biotic and abiotic factors.

In an attempt to improve biomonitoring of marine pollution, we have developed two proteomic approaches here. In the first portion of the thesis, a proteomic analysis was performed on peroxisomes isolated from mussels exposed either to one of three model anthropogenic pollutants, or two different types of crude oil, or from mussels exposed to the Prestige oil spill. Application of two-dimensional electrophoresis (2-DE) provided protein expression signatures (PES) for exposure to these different pollutants.Furthermore, several individual protein components of these PES could be putatively identified.

In the second portion of this work, such analysis of subproteomes was developed further in order to improve the applicability of this approach to biomonitoring. A simple fractionation procedure in combination with liquid chromatography and 2-DE provided samples from mussels residing in different regions of a pollution gradient around the harbor of Gothenburg, as well as from mussels exposed to two types of fuel oil similar to that of the Prestige that were suitable for environmental proteomics. In addition, we constructed a model for this approach that can be cross-validated in the future and applied to assess sources of fuel oil pollution in connection with biomonitoring programs.

Place, publisher, year, edition, pages
Stockholm: Department of Biochemistry and Biophysics, Stockholm University, 2009. 58 p.
proteomics, mussels, peroxisomes, two-dimensional electrophoresis (2-DE), difference gel electrophoresis (DIGE), bisphenol A (BPA), diallyl phthalate (DAP), 2, 2′, 4, 4′-tetrabromodiphenyl ether (PBDE-47), fuel oil, Prestige oil spill
National Category
Environmental Sciences Pharmacology and Toxicology
Research subject
urn:nbn:se:su:diva-26150 (URN)978-91-7155-801-5 (ISBN)
Public defence
2009-04-17, Magnélisalen , Kemiska övningslaboratoriet, Svante Arrhenius väg 12A, Stockholm University, Stockholm, 14:00 (English)
Available from: 2009-03-26 Created: 2009-03-10 Last updated: 2009-04-02Bibliographically approved

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Apraiz, ItxasoCristobal, Susana
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