Molt-inhibiting hormone immunoreactive neurons in the eyestalk neuroendocrine system of the blue crab, Callinectes sapidus.
2001 (English)In: Arthropod structure & development, ISSN 1467-8039, E-ISSN 1873-5495, Vol. 30, no 1, 69-76 p.Article in journal (Refereed) Published
The production of ecdysteroid molting hormones by crustacean Y-organs is negatively regulated by a neuropeptide, molt-inhibiting hormone. It is generally agreed that molt-inhibiting hormone is produced and released by the eyestalk neuroendocrine system. In the present study, immunocytochemical methods were used to detect molt-inhibiting hormone immunoreactive neurons in eyestalk ganglia of the blue crab, Callinectes sapidus. The primary antiserum used was generated against molt-inhibiting hormone of the green shore crab, Carcinus maenas. A preliminary Western blot analysis indicated the antiserum binds molt-inhibiting hormone of Callinectes sapidus. Using confocal and conventional immunofluorescence microscopy, molt-inhibiting hormone immunoreactivity was visualized in whole mounts and thin sections of Callinectes sapidus eyestalk ganglia. Immunoreactivity was detected in 15-25 neurosecretory cell bodies in the medulla terminalis X-organ, their associated axons and collateral branches, and their axon terminals in the neurohemal sinus gland. The cellular organization of molt-inhibiting hormone immunoreactive neurons in blue crabs is generally similar to that reported for other crab species. The combined results suggest the cellular structure of the molt-inhibiting hormone neuroendocrine system is highly conserved among brachyurans.
Place, publisher, year, edition, pages
2001. Vol. 30, no 1, 69-76 p.
Molt-inhibiting hormone; X-organ; Sinus gland; Neurosecretory cell; Neuroendocrine; Callinectes sapidus
IdentifiersURN: urn:nbn:se:su:diva-26637DOI: 10.1016/S1467-8039(01)00024-XPubMedID: 18088946OAI: oai:DiVA.org:su-26637DiVA: diva2:210788