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Enkephalinergic control of the secretory activity of neurons producing stereoisomers of crustacean hyperglycemic hormone in the eyestalk of the crayfish Orconectes limosus.
Laboratoire Signaux Endocrines et Toxines d'Invertébrés, CNRS EP2028, Université Pierre et Marie Curie-Ecole normale supérieure, 75230 Paris, France.
Institute of Zoophysiology, University of Bonn, Germany.ORCID iD: 0000-0001-7815-4868
Laboratoire Signaux Endocrines et Toxines d'Invertébrés, CNRS EP2028, Université Pierre et Marie Curie-Ecole normale supérieure, 75230 Paris, France.
Laboratoire Signaux Endocrines et Toxines d'Invertébrés, CNRS EP2028, Université Pierre et Marie Curie-Ecole normale supérieure, 75230 Paris, France.
2002 (English)In: Journal of Comparative Neurology, ISSN 0021-9967, E-ISSN 1096-9861, Vol. 444, no 1, 1-9 p.Article in journal (Refereed) Published
Abstract [en]

A subgroup of neurons in the classical X organ sinus gland neuroendocrine system of the crayfish (Orconectes limosus) eyestalk produces two chiral forms of the crustacean hyperglycemic hormone (CHH) in two different types of neurons: CHH in 22 cells and D Phe(3) CHH in eight cells. Previous reports have demonstrated that release of CHH from the sinus gland is inhibited by enkephalins. Here, we have addressed the questions of 1) whether this inhibition affects one or both types of CHH neurons, 2) where the site of enkephalinergic control of CHH and/or D Phe(3) CHH is, and 3) whether the inhibitory effect is due to direct or indirect interactions of enkephalinergic neurons with CHH cells. In vitro incubations of neurosecretory complexes followed by immunoassays of CHH isoforms indicated that both methionine and leucine enkephalins inhibit release of the two CHH isoforms from crayfish eyestalks, by a receptor mediated process. Whole mount double or triple immunofluorescence labelings combined with confocal microscopy revealed enkephalin immunostaining in all neuropils of the eyestalk, except in the sinus gland. Virtual thin confocal sections showed many close appositions between terminals of enkephalinergic neurons and dendritic arborizations of specific CHH immunoreactive cells in the medulla terminalis neuropil. This provides the first evidence for direct inputs from enkephalinergic neurons into dendrites of both CHH cell types, which suggests that enkephalins inhibit release of both CHH isoforms via synaptic contacts.

Place, publisher, year, edition, pages
2002. Vol. 444, no 1, 1-9 p.
Identifiers
URN: urn:nbn:se:su:diva-26642PubMedID: 11835178OAI: oai:DiVA.org:su-26642DiVA: diva2:210792
Available from: 2009-04-06 Created: 2009-04-06 Last updated: 2014-10-13Bibliographically approved

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