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Effects on Guppy brain aromatase activity following short-term steroid and 4-Nonyl phenol exposures
Stockholm University, Faculty of Science, Department of Zoology. (HOL)
Södertörns högskola. (HOL)
2010 (English)In: Environmental Toxicology, ISSN 1520-4081, E-ISSN 1522-7278, Vol. 25, no 3, 261-271 p.Article in journal (Refereed) Accepted
Abstract [en]

Brain oestrogen production, performed by the enzyme aromatase, can be disrupted/affected in teleost fish exposed to endocrine disruptors found in polluted aquatic environments. The guppy (Poecilia reticulata) was previously studied and confirmed to suffer negative effects on reproductive behaviours following inhibition of the brain aromatase reaction. Here Adult guppies (Poecilia reticulata) of both genders were subjected to known endocrine disruptors: the androgen androstenedione (A), the synthetic oestrogen 17α-ethynylestradiol (EE2) and the oestrogenic surfactant 4-nonylphenol (NP), at high (50 μg/L) and at environmentally relevant concentrations (10 ng/L EE2, 5 μg/L NP and 0.7 μg/L A) for two weeks followed by measurements of brain aromatase activity (bAA). In the adult males, bAA was stimulated by A and EE2 at 50 μg/L. Female activity was also stimulated by the higher oestrogenic treatment. At environmentally relevant concentrations only the EE2 treatment affected bAA, and only in males. The alkylphenolic substance NP produced no effect in either of the experiments, not on males nor females. The results indicate that short-term steroid treatments have stimulatory effects on guppy brain aromatase even at concentrations that can be found in the environment. We thus suggest bAA of adult guppies to be a suitable bioindicator of endocrine disruptors.

Place, publisher, year, edition, pages
2010. Vol. 25, no 3, 261-271 p.
Keyword [en]
Teleost brain, endocrine disruption, bio-indicator, 17α-ethynylestradiol, androstenedione
National Category
Zoology
Research subject
Functional Zoomorphology; Ecotoxicology
Identifiers
URN: urn:nbn:se:su:diva-26904DOI: 10.1002/tox.20494OAI: oai:DiVA.org:su-26904DiVA: diva2:211719
Projects
Aromatase and androgens in fish reproductive behaviour
Available from: 2009-04-20 Created: 2009-04-17 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Brain Aromatase in the guppy, Poecilia reticulata: Distribution, control and role in behaviour
Open this publication in new window or tab >>Brain Aromatase in the guppy, Poecilia reticulata: Distribution, control and role in behaviour
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Oestrogens are produced by aromatisation of androgens by the aromatase enzyme. In the vertebrate brain this synthesis has vital functions in nerve protection, cell proliferation and nerve development during injury respectively brain development. Brain oestrogens are also crucial in activating certain reproductive and aggressive behaviours in mammals and birds. Teleosts have remarkably high activity of brain aromatase (bAA) compared to mammals and birds; 100-1000 times higher in brain regions like the hypothalamus, pre-optic area and optic tectum. The role of brain oestrogens in teleost behaviour is, however, less clear than in e.g. songbirds and rodents. This thesis studies the potential role of brain aromatase and brain oestrogens in the reproductive behaviour of the guppy male (Poecilia reticulata), how guppy brain aromatase responds to steroids and is distributed in the adult brain.

The thesis shows that male behaviour can be affected by brain aromatase. Reduction of bAA by aromatase inhibitor treatment reduced the sexual behaviours sigmoid display and gonopodium swinging (I) and oestrogen receptor blocking with an oestrogen antagonist reduced the number of successful mating attempts (II). The anatomical study (IV) showed that brain aromatase is distributed in areas of the adult guppy brain that are connected to reproductive control and behaviour in vertebrates.

Guppy bAA is stimulated by both androgens and oestrogens (III) but is more sensitive to oestrogens, especially in males, and could thus be used as an indicator of endocrine disruption at low concentrations found in the environment.

The thesis can also conclude that females possess more brain aromatase than males, and that although it is expressed in the same pattern throughout the brain in both genders the enzymatic activity is differently distributed between the sexes.

Place, publisher, year, edition, pages
Stockholm: Department of Zoology, Stockholm University, 2009. 41 p.
Series
Södertörn Doctoral Dissertations, ISSN 1652-7399 ; 36
Keyword
Oestrogen production, behaviour, anatomy, endocrinology, bio markers, endocrine disruption
National Category
Microbiology
Research subject
Functional Zoomorphology
Identifiers
urn:nbn:se:su:diva-26942 (URN)978-91-86069-03-2 (ISBN)978-91-7155-870-1 (ISBN)
Public defence
2009-05-26, MA624, Södertörns högskola, Alfred Nobels allé 7, Huddinge, 10:00 (English)
Opponent
Supervisors
Projects
Aromatase and androgens in fish reproductive behaviour
Available from: 2009-05-05 Created: 2009-04-20 Last updated: 2009-06-09Bibliographically approved

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