Flavivirus NS5 associates with host-cell proteins zonula occludens-1 (ZO-1) and regulating synaptic membrane exocytosis-2 (RIMS2) via an internal PDZ binding mechanism
2009 (English)In: Biological chemistry (Print), ISSN 1431-6730, Vol. 390, 319-323 p.Article in journal (Refereed) Published
Dengue virus (DENV) and tick-borne encephalitis virus (TBEV) are flaviviruses, which can cause lethal hemorrhagic fever and encephalitis, respectively. Here, we demonstrate that the TBEV-NS5 and DENV-NS5 proteins use an internal binding mechanism to target human PDZ proteins. TBEV-NS5 has high affinity to regulating synaptic membrane exocytosis-2 (RIMS2) and Scribble, whereas DENV-NS5 binds primarily to the tight junction protein zonula occludens-1 (ZO-1). Targeting of TBEVNS5 to the plasma membrane is stabilised by ZO-1; however, DENV-NS5 co-localises with ZO-1 in the nucleus. These interactions have potential important roles in the ability of flaviviruses to manipulate cell proliferation, junction permeability and the interferon pathways.
Place, publisher, year, edition, pages
Berlin: Walter de Gruyter , 2009. Vol. 390, 319-323 p.
Research subject Molecular Genetics
IdentifiersURN: urn:nbn:se:su:diva-27644DOI: 10.1515/BC.2009.041ISI: 000265160000005OAI: oai:DiVA.org:su-27644DiVA: diva2:216714