Implications of N-terminal sequence elements in S. cerevisiae ribosomal protein L5
(English)Manuscript (Other academic)
Yeast ribosomal protein L5 (YrpL5) is an essential 5S rRNA-binding protein that forms the central protuberance of the large ribosomal subunit. Formation of the binary rpL5.5S rRNA complex is a prerequisite for nuclear import of rpL5 and for ribosome assembly. The involvment of the N-terminal sequences of YrpL5 in 5S rRNA interaction and nuclear import was studied by mutagenesis and functional complementation in S. cerevisiae. Furthermore, the ability of YrpL5 orthologous proteins from M. musculus (MrpL5), D. melanogaster (DrpL5) and A. thaliana (ArpL5) were non-functional in yeast cells. Nuclear import of YrpL5 requires conserved sequence elements in the N-terminus. Despite the presence of these elements in ArpL5, this protein was not recognized by the nuclear import machinery in yeast. This failure was probably due to lack of stable complex formation with yeast 5S rRNA.
Functional complementation, Mutation analysis, Ribosomal protein L5, nuclear localization, S. cerevisiae
Biochemistry and Molecular Biology
Research subject Cellbiology
IdentifiersURN: urn:nbn:se:su:diva-27726OAI: oai:DiVA.org:su-27726DiVA: diva2:217438