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Improved enzymatic activity of Thermomyces lanuginosus lipase immobilized in a hydrophobic particulate
YKI, Institute for Surface Chemistry.
Stockholm University, Faculty of Science, Department of Physical, Inorganic and Structural Chemistry. (Materials Chemistry Research Group)
Stockholm University, Faculty of Science, Department of Physical, Inorganic and Structural Chemistry. (Materials Chemistry Research Group)
YKI, Institute for Surface Chemistry.
2010 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 343, no 1, 359-365 p.Article in journal (Refereed) Published
Abstract [en]

Lipase from Thermomyces lanuginosus has been immobilized within particulate mesoporous silica carriers, with either hydrophilic or hydrophobic supporting surfaces, produced by the newly developed emulsion and solvent evaporation (ESE) method.  The Michaelis-Menten model was used to calculate the parameters related to the enzymatic activity of lipase i.e. the turnover number, kcat, and the specific activity. The specific activity was improved by immobilization of lipase onto the hydrophobic support, compared to lipase immobilized onto the hydrophilic support and lipase free in solution. The enhanced enzymatic activity of lipase onto a hydrophobic support was attributed to interfacial activation of the Thermomyces lanuginosus lipase when it is attached to a hydrophobic surface and a reduced denaturation. Confocal scanning laser microscopy (CLSM) studies, of fluorescently tagged lipase, showed that leakage of the lipase from the mesoporous particles was limited to an initial period of only a few hours. Both the rate and the amount of lipase leached were reduced when the lipase was immobilized onto the hydrophobic support.

Place, publisher, year, edition, pages
Elsevier Inc. , 2010. Vol. 343, no 1, 359-365 p.
Keyword [en]
Enzyme, lipase, mesoporous, silica, immobilization, hydrophobic, spheres, particles
National Category
Inorganic Chemistry Industrial Biotechnology Chemical Process Engineering Other Materials Engineering
Research subject
Inorganic Chemistry
Identifiers
URN: urn:nbn:se:su:diva-27826DOI: 10.1016/j.jcis.2009.11.014OAI: oai:DiVA.org:su-27826DiVA: diva2:218548
Projects
Synthesis, functionalisation and controlled release of mesoporous materials
Available from: 2009-05-20 Created: 2009-05-20 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Harnessing Mesoporous Spheres - transport studies and biotechnological applications
Open this publication in new window or tab >>Harnessing Mesoporous Spheres - transport studies and biotechnological applications
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Applications in controlled release and delivery calls for a good understanding of molecular transport within the carrier material and the dominating release mechanisms. It is clear that a better understanding of hindered transport and diffusion of guest molecules is important when developing new porous materials, e.g., surfactant templated silica spheres, for biotechnological applications. Confocal laser scanning microscopy was used to quantify the bulk release and intraparticle transport of small charged fluorescent dyes, and fluorescently-tagged neutral dextran, from mesoporous silica spheres. The time dependent release and the concentration profiles within the spheres have been used to analyze the release mechanisms using appropriate models. While the small, non-adsorbing anionic dye is released following a simple diffusion driven process, the concentration of the cationic dye varies radially within the spheres after loading. The release of the cationic dye is controlled by diffusion after an initial period of rapid release, which could be due to a significant fraction of the cationic dye that remains permanently attached to the negatively charged walls of the mesoporous silica spheres. The diffusion of dextran and the resulting flat concentration profiles could be related to the complex structural feature of the cylindrical pores close to the surface, and a possible conformational change of the dextran with the concentration. The stability and leaching of a catalytic enzyme, lipase, immobilized in hydrophobilized mesoporous support has also been quantified. Colloidal monodisperse mesoporous silica spheres were synthesized and transmission electron microscopy showed that the inner pore structure display a radially extending pores. The mesoporous spheres were used as solid supports for a lipid membrane incorporated with a multi-subunit redox-driven proton pump, which was shown to remain functional.

Place, publisher, year, edition, pages
Stockholm: Department of Physical, Inorganic and Structural Chemistry, Stockholm University, 2009. 69 p.
Keyword
Mesoporous, spheres, particles, CLSM, TEM, controlled-release, molecular transport, lipid membrane, enzyme, intraparticle, lipase, solid support
National Category
Inorganic Chemistry
Research subject
Materials Science
Identifiers
urn:nbn:se:su:diva-27797 (URN)978-91-7155-898-5 (ISBN)
Public defence
2009-09-02, Nordenskiöldsalen, Geovetenskapens hus, Svante Arrhenius väg 8 C, Stockholm, 10:00 (English)
Opponent
Supervisors
Projects
Synthesis, functionalisation and controlled release of mesoporous materials
Available from: 2009-05-27 Created: 2009-05-19 Last updated: 2009-05-20Bibliographically approved

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