A Membrane-reconstituted Multisubunit Functional Proton Pump on Mesoporous Silica Particles
2009 (English)In: ACS Nano, ISSN 1936-0851, Vol. 3, no 9, 2639-2646 p.Article in journal (Refereed) Published
We have investigated formation of a proteolipid membrane surrounding mesoporous silica particles with a diameter of 550 nm and pore sizes of 3.0 nm. A multisubunit redox-driven proton pump, cytochrome c oxidase, was incorporated into the membrane and we show that the enzyme is fully functional, both with respect to catalysis of O2 reduction to water, and charge separation across the membrane. The orientation of cytochrome c oxidase in the membrane was found to be the same (~70/30 %) in the lipid vesicles and in the silica-particle supported lipid membrane, which provides information on the mechanism by which the vesicles adsorb to the surface. Furthermore, cytochrome c oxidase could maintain a proton electrochemical gradient across the supported proteomembrane, i.e. the membrane system was proton tight, defining an interior particle compartment that is separated from the surrounding aqueous media. Such a biofunctional cellular interface, supported onto a colloid that has a connected interior cytoskeleton-like pore structure, provides a basis for functional studies of membrane-bound transport proteins, and also for applications within pharmaceutical drug delivery.
Place, publisher, year, edition, pages
2009. Vol. 3, no 9, 2639-2646 p.
Supported lipid bilayer, mesoporous spheres, nanoparticles, membrane protein, drug delivery, cytochrome c oxidase
Inorganic Chemistry Industrial Biotechnology Physical Chemistry
Research subject Biochemistry; Materials Science
IdentifiersURN: urn:nbn:se:su:diva-27829DOI: 10.1021/nn9005413ISI: 000269988600027OAI: oai:DiVA.org:su-27829DiVA: diva2:218562
ProjectsSynthesis, functionalisation and controlled release of mesoporous materials