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Development and reproduction of the freshwater harpacticoid copepod Attheyella crassa for assessing sediment-associated toxicity
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
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2007 (English)In: Aquatic Toxicology, ISSN 0166-445X, Vol. 83, no 3, 180-9 p.Article in journal (Refereed) Published
Abstract [en]

Both freshwater and marine sediments are sinks for many anthropogenic substances. This may pose a risk to benthic and epibenthic organisms and it is crucial that toxicity tests that are available for environmental risk assessment can identify potentially adverse effects of sediment-associated substances on benthic organisms, such as harpacticoid copepods. While marine harpacticoids have been protected via a number of acute and chronic sediment tests, the freshwater harpacticoid copepod community has so far been neglected in such activities. The main aim of the present study was therefore to (a) find a suitable freshwater harpacticoid copepod, (b) establish robust laboratory mass cultures and (c) develop a chronic test for assessment of sediment-associated toxicity using spiked sediments. After several cultivation trials with a number of potential test species, the choice fell on the benthic freshwater harpacticoid copepod Attheyella crassa, a species that possesses many of the characteristic features identified as prerequisites for toxicity test organisms, e.g. it has a sexual reproduction, it is relatively easy to grow and keep in mass cultures in the laboratory, and it has a small body size. Owing to the relatively long generation time of freshwater harpacticoids (in relation to many marine harpacticoids), it was decided that the test should be separated into a development part (21 days) and a reproduction part (14 days) running in parallel. As a reference substance we used the fungicide tebuconazole, which is currently subject to risk assessment and which partitions to soil and sediment. Clear concentration-related responses were observed for all endpoints analyzed. Nauplia body length was the most sensitive endpoint with a measured time weighted LOEC(water) of 20microg/L. The corresponding LOEC(water) for larval mortality and offspring production was 65 and 62microg/L, respectively. In conclusion, A. crassa is an ecologically relevant test species for freshwater ecosystems and particularly for the cold, oligotrophic and often acidic lakes of Northern Europe. Regardless of the relatively long generation time of this species, our results clearly show that sediment-associated toxicity related to development and sexual reproduction can be assessed within 2-3 weeks exposure with the developed bioassay.

Place, publisher, year, edition, pages
2007. Vol. 83, no 3, 180-9 p.
URN: urn:nbn:se:su:diva-29181DOI: 10.1016/j.aquatox.2007.04.002ISI: 000248277300003PubMedID: 17512064OAI: diva2:229433
Available from: 2009-08-12 Created: 2009-08-12 Last updated: 2009-08-13Bibliographically approved
In thesis
1. Development of Methods for the Analysis of Polar Compounds in Environmental Matrices using LC/UV and LC/MS
Open this publication in new window or tab >>Development of Methods for the Analysis of Polar Compounds in Environmental Matrices using LC/UV and LC/MS
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In recent years, the awareness of environmentally hazardous compounds with polar and ionizable properties has intensified. Pharmaceuticals, the pesticide tebuconazole as well as the artificial sweetener sucralose are examples of such new, emerging, contaminants. The toxicological and ecotoxicological effects of these contaminants require investigation. This has led to an increasing need for analytical methods capable of measuring polar compounds in environmental matrices.

 This thesis describes the use of liquid chromatography (LC) hyphenated with ultraviolet (UV) or mass spectrometric (MS) detection for four environmental applications. Paper I describes a method for the detection and qualitative analysis of hydroxyalkyl cobalamins using LC/MS. The method was part of a project for the determination of carcinogenic compounds bound as adducts to the DNA molecule. In paper II, a LC/UV method for the analysis of the pesticide tebuconazole was developed. The method was used to evaluate a novel ecotoxicological test system. Paper III describes an LC/MS method for the determination of the artificial sweetener sucralose in sewage and recipient water. The sucralose molecule and its fragments were found difficult to detect, and a method was developed as an alternative to multiple reaction monitoring (MRM). Both quadrupoles scanned the same ion, a novel technique hypothesized to reduce matrix effects compared to selected ion recording (SIR). Paper IV describes a method for the extraction and analysis of pharmaceuticals in sediment. The study emphasizes the evaluation of suitable solvents for the extraction of basic, neutral and acidic pharmaceuticals. It was concluded that an extraction solvent containing acetone : McIlvaine buffer (pH 4) was appropriate. Electrospray LC/MS in the MRM mode was used. Additional qualitative identification of non target pharmaceuticals was performed by accurate mass measurements utilizing a quadrupole time-of-flight (Q/TOF) instrument.

Place, publisher, year, edition, pages
Stockholm: Department of Applied Environmental Science (ITM), Stockholm University, 2009. 31 p.
National Category
Natural Sciences
Research subject
Applied Environmental Science
urn:nbn:se:su:diva-29108 (URN)978-91-7155-910-4 (ISBN)
Public defence
2009-09-11, De Geersalen, Geovetenskapens hus, Svante Arrhenius väg 8 A, Stockholm, 10:00 (Swedish)
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: Submitted.Available from: 2009-08-21 Created: 2009-08-11 Last updated: 2009-08-13Bibliographically approved

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Stiernström, SaraMinten, JohannaAdolfsson-Erici, MargarethaBengtsson, Bengt-ErikBreitholtz, Magnus
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