Structural determination of the O-antigenic polysaccharide from Escherichia coli O74
2009 (English)In: Carbohydrate Research, ISSN 0008-6215, Vol. 344, no 12, 1592-1595 p.Article in journal (Refereed) Published
The structure of the O-antigen polysaccharide (PS) from Escherichia coli O74 has been determined. Component analysis, together with 1H and 13C NMR spectroscopy as well as 1H,15N-HSQC experiments were employed to elucidate the structure. Inter-residue correlations were determined by 1H,1H-NOESY and 1H,13C-heteronuclear multiple-bond correlation experiments. The PS is composed of tetrasaccharide repeating units with the following structure:
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Cross-peaks of low intensity from an α-linked N-acetylglucosamine residue were present in the NMR spectra, and spectral analysis indicates that they originate from the penultimate residue in the polysaccharide. Consequently, the biological repeating unit has a 3-substituted N-acetyl-d-glucosamine residue at its reducing end. The 1H, 13C and 15N NMR chemical shifts of the α- and β-anomeric forms of d-Fucp3NAc are also reported. The repeating unit of the E. coli O74 O-antigen is identical to that of the capsular polysaccharide from E. coli K45.
Place, publisher, year, edition, pages
Elsevier Ltd. , 2009. Vol. 344, no 12, 1592-1595 p.
Escherichia coli; Lipopolysaccharide; Capsular polysaccharide; NMR; Biological repeating unit
Other Basic Medicine
Research subject Organic Chemistry
IdentifiersURN: urn:nbn:se:su:diva-29260DOI: 10.1016/j.carres.2009.03.023ISI: 000269734500028OAI: oai:DiVA.org:su-29260DiVA: diva2:232041