Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Determination of amino acids in size-segregated airborne particles using GC-HRMS
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
Stockholm University, Faculty of Science, Department of Meteorology .
(English)Manuscript (preprint) (Other academic)
Abstract [en]

A gas chromatography–high resolution mass spectrometry methodology for the simultaneous determination of 14 amino acids in size-segregated airborne particles has been developed. A five-stage high-volume low-pressure Berner Cascade Impactor was used to collect the particles on Tedlar® substrates and Millipore Fluoropore filters. The substrates and filters were extracted with methanol and the extract was treated with a cation exchanger to concentrate the amino acids. N-methyl-N-tert-butyldimethylsilyl-trifluoroacetamide (MTBSTFA)+1% t-butyldimethyl-chlorosilane (TBDMCS) and acetonitril were added to silylate the amino acids before determination with gas chromatography-high resolution mass spectrometry working in selective ion recording mode. The detection limits (S/N ³ 3) for most of the amino acids ranged from 1 to 3 pg amino acid injected. The recoveries for the entire procedure were in the range of 61 to 104% and the variations obtained were approximately 10% for all amino acids. The procedure has been applied to ambient atmospheric samples collected during the Arctic Ocean Expedition (AOE-2001) from July through August 2001, 71 to 90°N.

National Category
Environmental Sciences
Research subject
Applied Environmental Science
Identifiers
URN: urn:nbn:se:su:diva-29473OAI: oai:DiVA.org:su-29473DiVA: diva2:233461
Available from: 2009-09-01 Created: 2009-09-01 Last updated: 2010-01-14Bibliographically approved
In thesis
1. Development of GC-HRMS procedures for determination of naturally occuring polar compounds in various environmental applications 
Open this publication in new window or tab >>Development of GC-HRMS procedures for determination of naturally occuring polar compounds in various environmental applications 
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The overall objectives of this dissertation were to gain further understanding of the two following environmentally significant issues: (i) Determination of natural steroid hormones in blood plasma from perch (Perca fluviatilis) during a reproductive cycle and to evaluate the possibility of using the steroid composition as a biomarker for early signs of endocrine disruptive effects. (ii) Determination of dissolved free amino acids in size resolved airborne particles collected over the Arctic pack ice area (>80°N) in summer and to study their overall relationship with the ocean surface microlayer as a potential source.

 This was made possible by the development of two separate gas chromatographic–high resolution mass spectrometry methods. To enable separation with gas chromatography both steroids and amino acids had to be chemically modified to increase their volatility. The small sample volumes available and low concentrations of analytes required a multi-step clean-up procedure to enable determination.

 The results showed that the circulating levels of steroids in perch varied over the year and that the levels of some androgens were lower in female perch exposed to leachate from a refuse dump compare to unexposed perch, which may explain the decline in fertility observed for the former group. This also indicated that the steroid composition in the blood plasma may function as a sensitive biomarker.

 The levels of dissolved free amino acids were enriched in the submicrometer aerosol, peaking in sizes around 100nm aerodynamic diameter. These findings do support a previous assumption that the most likely source for these particles is the surface microlayer of the open water between the ice floes. The most likely exchange mechanism of biogenic matter between ocean and atmosphere is the bursting of bubbles at the surface of the leads. This mechanism would provide a very important and poorly understood link between cloud radiative properties and marine biochemistry in the summer high Arctic (>80°N) through the production of cloud condensation nuclei.

Place, publisher, year, edition, pages
Stockholm: Department of Applied Environmental Science (ITM), Stockholm University, 2009. 57 p.
Keyword
GC-HRMS, steroid hormones, amino acids, perch, size-resolved aerosol, Arctic
National Category
Natural Sciences
Research subject
Applied Environmental Science
Identifiers
urn:nbn:se:su:diva-29283 (URN)978-91-7155-907-4 (ISBN)
Public defence
2009-09-25, De Geer salen, Geovetenskapens hus, Svante Arrhenius väg 14, Stockholm, 10:00 (English)
Opponent
Supervisors
Note
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 1: Accepted. Paper 4: In progress. Paper 5: In progress.Available from: 2009-09-03 Created: 2009-08-20 Last updated: 2009-09-01Bibliographically approved

Open Access in DiVA

No full text

Other links

Link to doctoral thesis

Search in DiVA

By author/editor
Widell, BodilLeck, Caroline
By organisation
Department of Applied Environmental Science (ITM)Department of Meteorology
Environmental Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 153 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf