Determination of amino acids in size-segregated airborne particles using GC-HRMS
(English)Manuscript (preprint) (Other academic)
A gas chromatography–high resolution mass spectrometry methodology for the simultaneous determination of 14 amino acids in size-segregated airborne particles has been developed. A five-stage high-volume low-pressure Berner Cascade Impactor was used to collect the particles on Tedlar® substrates and Millipore Fluoropore filters. The substrates and filters were extracted with methanol and the extract was treated with a cation exchanger to concentrate the amino acids. N-methyl-N-tert-butyldimethylsilyl-trifluoroacetamide (MTBSTFA)+1% t-butyldimethyl-chlorosilane (TBDMCS) and acetonitril were added to silylate the amino acids before determination with gas chromatography-high resolution mass spectrometry working in selective ion recording mode. The detection limits (S/N ³ 3) for most of the amino acids ranged from 1 to 3 pg amino acid injected. The recoveries for the entire procedure were in the range of 61 to 104% and the variations obtained were approximately 10% for all amino acids. The procedure has been applied to ambient atmospheric samples collected during the Arctic Ocean Expedition (AOE-2001) from July through August 2001, 71 to 90°N.
Research subject Applied Environmental Science
IdentifiersURN: urn:nbn:se:su:diva-29473OAI: oai:DiVA.org:su-29473DiVA: diva2:233461