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Gonad development and plasma steroid profiles by HRGC/HRMS during one reproductive cycle in reference and leachate-exposed female perch (Perca fluviatilis)
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
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2004 (English)In: Toxicology and Applied Pharmacology, ISSN 0041-008X, E-ISSN 1096-0333, Vol. 195, no 2, 247-261 p.Article in journal (Refereed) Published
Abstract [en]

Endocrine processes were studied in reference female perch (Perca fluviatilis) from Lake Djursjön and in leachate-exposed females from Lake Molnbyggen during one reproductive cycle. A high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) technique enabled the analysis of circulating sex steroids [progesterone (P), 17α-hydroxyprogesterone (17α-OHP), androstenedione (A), testosterone (T), estrone (E1), and 17β-estradiol (E2)], the maturation-inducing hormone (MIH) 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-P), and glucocorticoids [11-deoxycortisol (11-DC) and cortisol]. Body and organ measurements, ratio of sexually mature (SM) females, and frequencies of body lesions, were also recorded. High frequencies of body lesions and a low ratio of SM females were generally found in Molnbyggen. These females also had lower gonadosomatic index (GSI) and liver-somatic index (LSI) in the early stages of vitellogenesis, indicating either a delayed onset of oogenesis or an impaired vitellogenesis. Non-reproducing females from both lakes showed a similar LSI cycle as SM females. Increasing T and A levels in SM females from Djursjön coincided with the GSI peak at the time of spawning. These two androgens were on several occasions lower in SM females from Molnbyggen, possibly the result of a disrupted 17,20-lyase activity of the P450 17α enzyme because only minor effects were observed on circulating P and 17α-OHP levels. E2 and E1 levels increased during vitellogenesis in females from both lakes, with E1 levels more closely resembling the peaks of A and GSI. Peak levels of 17α,20β-P at the time of spawning confirmed its function as the MIH in perch, while the simultaneous peak of 11-DC suggests that it also may be involved in final maturation of the oocytes.

Place, publisher, year, edition, pages
2004. Vol. 195, no 2, 247-261 p.
Keyword [en]
Reproductive cycle; Gonad development; Sexual immaturity; Steroid profiles; Endocrine disruption; HRGC/HRMS; Refuse dump leachate; Perch (Perca fluviatilis)
National Category
Environmental Sciences
Research subject
Applied Environmental Science
Identifiers
URN: urn:nbn:se:su:diva-29475DOI: 10.1016/j.taap.2003.11.017OAI: oai:DiVA.org:su-29475DiVA: diva2:233475
Available from: 2009-09-01 Created: 2009-09-01 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Development of GC-HRMS procedures for determination of naturally occuring polar compounds in various environmental applications 
Open this publication in new window or tab >>Development of GC-HRMS procedures for determination of naturally occuring polar compounds in various environmental applications 
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The overall objectives of this dissertation were to gain further understanding of the two following environmentally significant issues: (i) Determination of natural steroid hormones in blood plasma from perch (Perca fluviatilis) during a reproductive cycle and to evaluate the possibility of using the steroid composition as a biomarker for early signs of endocrine disruptive effects. (ii) Determination of dissolved free amino acids in size resolved airborne particles collected over the Arctic pack ice area (>80°N) in summer and to study their overall relationship with the ocean surface microlayer as a potential source.

 This was made possible by the development of two separate gas chromatographic–high resolution mass spectrometry methods. To enable separation with gas chromatography both steroids and amino acids had to be chemically modified to increase their volatility. The small sample volumes available and low concentrations of analytes required a multi-step clean-up procedure to enable determination.

 The results showed that the circulating levels of steroids in perch varied over the year and that the levels of some androgens were lower in female perch exposed to leachate from a refuse dump compare to unexposed perch, which may explain the decline in fertility observed for the former group. This also indicated that the steroid composition in the blood plasma may function as a sensitive biomarker.

 The levels of dissolved free amino acids were enriched in the submicrometer aerosol, peaking in sizes around 100nm aerodynamic diameter. These findings do support a previous assumption that the most likely source for these particles is the surface microlayer of the open water between the ice floes. The most likely exchange mechanism of biogenic matter between ocean and atmosphere is the bursting of bubbles at the surface of the leads. This mechanism would provide a very important and poorly understood link between cloud radiative properties and marine biochemistry in the summer high Arctic (>80°N) through the production of cloud condensation nuclei.

Place, publisher, year, edition, pages
Stockholm: Department of Applied Environmental Science (ITM), Stockholm University, 2009. 57 p.
Keyword
GC-HRMS, steroid hormones, amino acids, perch, size-resolved aerosol, Arctic
National Category
Natural Sciences
Research subject
Applied Environmental Science
Identifiers
urn:nbn:se:su:diva-29283 (URN)978-91-7155-907-4 (ISBN)
Public defence
2009-09-25, De Geer salen, Geovetenskapens hus, Svante Arrhenius väg 14, Stockholm, 10:00 (English)
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Note
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 1: Accepted. Paper 4: In progress. Paper 5: In progress.Available from: 2009-09-03 Created: 2009-08-20 Last updated: 2009-09-01Bibliographically approved

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