Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A natriuretic peptide is formed at high levels in actinorhizal nodules of Alnus glutinosa
Stockholm University, Faculty of Science, Department of Botany.
Albrecht von Haller Institute for Plant Sciences, Plant Biochemistry, Göttingen University, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany .
Albrecht von Haller Institute for Plant Sciences, Plant Biochemistry, Göttingen University, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany .
Department of Molecular Biology, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands.
Show others and affiliations
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Plant homologues of mammalian natriuretic peptides (Plant Natriuretic Peptides, PNPs, also called γ-expansins as they represent expansins lacking the third exon/C-terminal domain) are small apoplastic proteins that have been associated with various biotic and abiotic stresses. Here, the characterization of PNP from Alnus glutinosa is described that is expressed at high levels in actinorhizal root nodules induced by nitrogen-fixing Frankia, and at very low levels during fruit development. In order to determine the function of this PNP in nodules, the cDNA was expressed in transgenic tobacco under control of the CaMV35S promoter. The results reveal a very slight increase in resistance to oxidative/nitrosative stress. Based on the occurrence of PNPs in different types of nitrogen-fixing root nodules and the oxidative/nitrosative stress these nodule types are exposed to, we hypothesize that Ag67 is involved in the response to nitric oxide.

Keyword [en]
Frankia, actinorhiza, nodulin, ectopic expression
National Category
Botany
Research subject
Plant Physiology
Identifiers
URN: urn:nbn:se:su:diva-30844OAI: oai:DiVA.org:su-30844DiVA: diva2:274358
Available from: 2009-10-28 Created: 2009-10-28 Last updated: 2010-01-14
In thesis
1. Control of reactive oxygen species homeostasis in response to environmental stress
Open this publication in new window or tab >>Control of reactive oxygen species homeostasis in response to environmental stress
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Plants are exposed to various fluctuations in their environmental conditions - light intensity, temperature, water status - and have to adapt in order to survive. Plant acclimatory responses can include the formation of new tissues, e.g., aerenchyma, or the activation of defense systems, e.g., the ascorbate-glutathione cycle for detoxification of reactive oxygen species (ROS). A prominent ROS is hydrogen peroxide (H2O2), a non-radical molecule formed during the reduction of oxygen. Due to its non-radical nature, H2O2 is more stable than other ROS and this longevity makes it the most abundant ROS in the plant cell and potentially harmful. In spite of this, H2O2 is involved in several signal transduction processes in plant cells, e.g., in the control of stomatal aperture, in plant-symbiont- and in plant-pathogen interactions, also in programmed cell death (PCD). Therefore, it is important for plant cells to maintain a tight control of H2O2 levels.

In this study, the role of H2O2 production and -detoxification was studied in different plant processes. First, signaling leading to aerenchyma formation was studied in Arabidopsis thaliana. This plant shows lysigenous aerenchyma formation, a process involving PCD, which meant it was preceded by H2O2 formation. Second, the role of a H2O2 detoxifying enzyme, cytosolic ascorbate peroxidase 2 (APX2) from A. thaliana, in the local and systemic response to excess light stress, was studied by means of the characterization of APX2 knockout mutant lines. Third, antioxidant defense was studied in two types of nitrogen-fixing actinorhizal root nodules with different oxygen metabolism, from Datisca glomerata and Casuarina glauca. Fourth, the role of a plant natriuretic peptide from actinorhizal nodules of Alnus glutinosa, in abiotic stress resistance was studied.

Place, publisher, year, edition, pages
Stockholm: Department of Botany, Stockholm University, 2009. 51 p.
Keyword
Stress, antioxidant defense, ascorbate peroxidase, aerenchyma, actinorhiza
National Category
Botany
Research subject
Plant Physiology
Identifiers
urn:nbn:se:su:diva-30849 (URN)978-91-7155-967-8 (ISBN)
Public defence
2009-11-27, Föreläsningssalen, Botanicum, Lilla Frescativägen 5, Stockholm, 13:00 (English)
Opponent
Supervisors
Note
At the time of the doctoral defence, the following papers were unpublished and had a status as follows: Paer 2: Manuscript. Paper 3: Manuscript. Paper 4: Manuscript.Available from: 2009-11-05 Created: 2009-10-28 Last updated: 2009-10-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Link to doctoral thesis

Search in DiVA

By author/editor
Plaszczyca, MalgorzataPawlowski, Katharina
By organisation
Department of Botany
Botany

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 59 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf