Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Sodium induces simultaneous changes in cytosolic calcium and pH in salt-tolerant quince protoplasts
University of Pisa, Italy.
Stockholm University, Faculty of Science, Department of Botany. (Sylvia Lindberg)
2009 (English)In: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 166, 1755-1763 p.Article in journal (Refereed) Published
Abstract [en]

Previousexperimentswithsalt-resistantquinceBA29(Cydonia oblonga cv.Mill.) have shownthatthiscultivartakesupsodiumtransientlyintothecytosolofshoot protoplasts onlyintheabsenceofcalciumchloride,orat o1mMcalciumchloride. Addition ofNaClZ100mMtosingleprotoplastsfrom in vitro-cultivated quinceinthe presence of1.0mMcalciuminducedinstantchangesinthecytosolicconcentrations of calciumandprotons.Thesechangeswereinvestigatedbyuseoftetra [acetoxymethyl] estersofthefluorescentstilbenechromophoresFura2andbis- carboxyethyl-carboxyfluorescein (BCECF),respectively.ThecytosolicCa2+ dynamics in theprotoplastsweredependentontheconcentrationofNaCladded.Thechanges in calciumdifferedinamplitudeandfinalconcentrationandwerecorrelatedintime mainly withchangesinpH.Additionof100–400mMNaCltotheprotoplastscausedan oscillating increaseinthecytosoliclevelofcalcium,andthenadecrease.Addition of mannitol,ofequiosmolarconcentrationtoNaCl,didnotincreasethecytosolic calcium concentration.Moreover,therewasnoincreaseincytosoliccalciumwhen NaCl wasaddedinthepresenceofcalciumbindingethyleneglycol-bis(beta- aminoethylether)-N,N,N0,N0-tetra aceticacid(EGTA),orlantanorverapamil,two inhibitors ofplasmamembranecalciumchannels.Therefore,weconcludethat,in salt-resistant quince,sodiuminducesaninfluxofcalciumintothecytosolbyplasma membrane calciumchannels,andasimultaneousincreaseincytosolicpH.Because these changeswereobtainedinthepresenceof1mMcalciuminthemedium,they were notduetosodiumuptakeintothecytosol.

Place, publisher, year, edition, pages
GmbH: Elsevier , 2009. Vol. 166, 1755-1763 p.
Keyword [en]
cytosolic calcium and pH; fluorescence microscopy; Quince; salinity; sodium
National Category
Microbiology
Research subject
Physiological Botany
Identifiers
URN: urn:nbn:se:su:diva-32349DOI: 10.1016/j.jplph.2009.05.006ISI: 000271524900006OAI: oai:DiVA.org:su-32349DiVA: diva2:280092
Projects
Salinity stress and crop responses
Available from: 2009-12-08 Created: 2009-12-08 Last updated: 2017-12-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Lindberg, Sylvia
By organisation
Department of Botany
In the same journal
Journal of plant physiology (Print)
Microbiology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 46 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf