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Distinct expression of muscle-specific microRNAs (myomirs) in brown adipocytes.
Stockholm University, Faculty of Science, The Wenner-Gren Institute , Department of Physiology. Stockholm University, Faculty of Science, The Wenner-Gren Institute .
Stockholm University, Faculty of Science, The Wenner-Gren Institute , Department of Physiology. Stockholm University, Faculty of Science, The Wenner-Gren Institute .
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2009 (English)In: Journal of Cellular Physiology, ISSN 0021-9541, E-ISSN 1097-4652, Vol. 218, no 2, 444-449 p.Article in journal (Refereed) Published
Abstract [en]

MicroRNAs, a novel class of post-transcriptional gene regulators, have been demonstrated to be involved in several cellular processes regulating the expression of protein-coding genes. Here we examine murine white and brown primary cell cultures for differential expression of miRNAs. The adipogenesis-related miRNA miR-143 was highly expressed in mature white adipocytes but was low in mature brown adipocytes. Three classical "myogenic" miRNAs miR-1, miR-133a and miR-206 were absent from white adipocytes but were specifically expressed both in brown pre- and mature adipocytes, reinforcing the concept that brown adipocytes and myocytes derive from a common cell lineage that specifies energy-dissipating cells. Augmentation of adipocyte differentiation status with norepinephrine or rosiglitazone did not affect the expression of the above miRNAs, the expression levels of which were thus innately regulated. However, expression of the miRNA miR-455 was enhanced during brown adipocyte differentiation, similarly to the expression pattern of the brown adipocyte differentiation marker UCP1. In conclusion, miRNAs are differentially expressed in white and brown adipocytes and may be important in defining the common precursor cell for myocytes and brown adipocytes and thus have distinct roles in energy-storing versus energy-dissipating cells.

Place, publisher, year, edition, pages
2009. Vol. 218, no 2, 444-449 p.
URN: urn:nbn:se:su:diva-32905DOI: 10.1002/jcp.21621ISI: 000262270600025PubMedID: 18937285OAI: diva2:281916
Available from: 2009-12-17 Created: 2009-12-17 Last updated: 2010-04-12Bibliographically approved
In thesis
1. Regulatory Factors that Reveal Three Distinct Adipocytes: The Brown, the White and the Brite
Open this publication in new window or tab >>Regulatory Factors that Reveal Three Distinct Adipocytes: The Brown, the White and the Brite
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Adipose tissues have long been considered to derive from a common origin. Even the functionally different brown and white adipose tissues were generalized to share a common origin. Brown adipose tissue is a highly innervated and vascularised tissue containing multilocular and multimitochondrial brown adipocytes. Brown adipose tissue expends energy through sympathetic nervous system-mediated non-shivering thermogenesis, where uncoupling protein 1 (UCP1) is the key player. In contrast, white adipose tissue consists of unilocular white adipocytes with a main role to store energy in the form of the lipid droplet.

We know today that this generalisation is exaggerated since adipocytes can derive from more than one origin and not only be brown or white. We and others have demonstrated that the brown adipocyte has a dermomyotomal origin and derives from the adipomyocyte, the precursor cell that can also become a myocyte, whereas white adipocytes are suggested to derive from pericytes, cells that are embedded within the vascular vessel walls. For a long time there has been evidence that energy-expending adipocytes reside within certain white adipose tissues, based on the fact that cold exposure, by switching on the sympathetic nervous system, leads to levels of UCP1 that are not detectable in mice housed at thermoneutrality. We demonstrated that these cells have a molecular signature that is distinct from brown and white adipocytes. Since these energy-expending cells reside within certain white adipose tissues, we chose to name them brite (brown in white) adipocytes. Moreover, we also identified regulatory factors that were specifically expressed in each adipocyte type, thus, facilitating the possibility to identify the three adipocytes: the brown, the white and the brite.

Place, publisher, year, edition, pages
Stockholm: The Wenner-Gren Institute, Stockholm University, 2010. 89 p.
adipocyte, UCP1, brite, brown, white, Hox Zic, miRNA
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urn:nbn:se:su:diva-38362 (URN)978-91-7447-048-2 (ISBN)
Public defence
2010-05-12, sal E306, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 10:00 (English)
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 4: Manuscript. Paper 5: Manuscript. Available from: 2010-04-20 Created: 2010-04-09 Last updated: 2010-04-22Bibliographically approved

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Walden, Tomas BNedergaard, JanCannon, Barbara
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