Analytical protocol for identification of BMAA and DAB in biologicalsamples
2010 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 135, 127-132 p.Article in journal (Refereed) Published
-N-methylamino-L-alanine (BMAA) is a non-protein amino acid, thought to be inflicting neurodegenerative diseases related to ALS/PDC in human beings. Due to conflicting data concerning the presence of BMAA in various biological matrixes, we present a robust and sensitive method for high confidence identification of BMAA after derivatization by 6-aminoquinolyl-N
-hydroxysuccinimidyl carbamate (AQC). The efficient sample pretreatment in combination with LC-MS/MS SRM enables chromatographic separation of BMAA from the isomer 2,3-diaminobutyric acid (DAB). The method is applicable for selective BMAA/DAB detection in various biological samples ranging from a prokaryotic cyanobacterium to eukaryotic fish.
Place, publisher, year, edition, pages
The Royal Society of Chemistry , 2010. Vol. 135, 127-132 p.
IdentifiersURN: urn:nbn:se:su:diva-33260DOI: 10.1039/b921048bOAI: oai:DiVA.org:su-33260DiVA: diva2:282781