Possibilities and limitations of equilibrium sampling using polydimethylsiloxane in fish tissue.
2009 (English)In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 77, no 6, 764-70 p.Article in journal (Refereed) Published
Polydimethylsiloxane (PDMS) has been used for passive equilibrium sampling in numerous abiotic environmental matrices. Recently, this approach was extended to lipid-rich tissue. This work investigated the possibilities and limitations of using PDMS thin-film extraction for in tissue equilibrium sampling in fish species of varying lipid content. Polychlorinated biphenyls (PCBs) were used as model lipophilic organic pollutants. PDMS thin-films were inserted in intact fish tissue for differing time periods (1h up to 1 week). The thin-films were then solvent-extracted and the extracts were analyzed using gas chromatography coupled to mass spectrometry. Whether equilibrium had been established was investigated either by using PDMS thin-films of multiple thicknesses (140-620 microm) or by assessing kinetics by means of time series. Equilibration was found to be rapid (i.e. in the range of hours) in lipid-rich fish whereas equilibrium was not achieved within one week in tissues with low or medium lipid content (i.e. up to 2% lipids). Regarding lipid-rich fish, the newly developed method was found to be sufficiently sensitive to determine equilibrium partitioning concentrations of PCBs in lipids of samples from the Baltic Sea, and it is a promising approach for any kind of fatty tissue.
Place, publisher, year, edition, pages
2009. Vol. 77, no 6, 764-70 p.
In tissue equilibrium sampling, Silicone thin-film extraction, Internal exposure, Solid Phase Microextraction (SPME), Persistent organic pollutants (POPs), Environmental monitoring
Environmental Sciences Meteorology and Atmospheric Sciences
IdentifiersURN: urn:nbn:se:su:diva-34613DOI: 10.1016/j.chemosphere.2009.08.025ISI: 000271301300009PubMedID: 19765800OAI: oai:DiVA.org:su-34613DiVA: diva2:285293