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Differential neuronal expression of three Drosophila ion transport peptide splice forms indicate multiple functions of peptidergic neurons
Stockholm University, Faculty of Science, Department of Zoology, Functional Morphology. Stockholm University, Faculty of Science, Department of Zoology. (Dircksen, Heinrich)ORCID iD: 0000-0001-7815-4868
Stockholm University, Faculty of Science, Department of Zoology, Functional Morphology. Stockholm University, Faculty of Science, Department of Zoology.
Stockholm University, Faculty of Science, Department of Zoology, Functional Morphology. Stockholm University, Faculty of Science, Department of Zoology.
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2009 (English)In: Comparative Biochemistry and Physiology A, ISSN 1095-6433, E-ISSN 1531-4332, Vol. 153A, no 2, suppl. 1, S79- p.Article in journal (Refereed) Published
Abstract [en]

We identified previously two long (DrmITPL1 and -L2) and one amidated short isoform (DrmITP) of insect ion transport peptides (ITPs) as products derived by alternatively splicing from the Drosophila itp-gene (CG13586). The peptides are members of a large family of arthropod neuropeptides incl. crustacean hyperglycemic hormones (CHH/ITP-family), but similar ITPs are only known in locusts to have antidiuretic bioactivity on the hindgut. We localised the peptides by in situ hybridisation and immunocytochemistry with isoform-specific antibodies in the nervous system of larval (L3) and adult Drosophila melanogaster and screened Gal4-lines specific for peptidergic cells. Four neurosecretory cells in brain-corpora cardiaca/allata putatively release DrmITP as a hormone in all stages. DrmITP also occurs in interneurons in the brain/ventral ganglia and in neurons efferent towards the hindgut. Some interneurons are identical to well-known circadian clock neurons for which the effector molecules were elusive but are responsible for the evening bouts of locomotor activity in flies. DrmITPL1 and -L2 were found only in peripheral lateral bipolar and putative sensory neurons which are likely to play a role in the control of growth, hindgut ion transport and heart beat. With DrmITP identified in brain neurosecretory cells, hindgut-innervating neurons in the abdominal ganglia and one pair in the abdomen close to the larval anal organ or innervating the adult rectal pads, both chloride-transporting organs, we are facing an enormous complexity in multiple functions of differentially expressed ITP/Ls derived from a single gene. Preliminary results using Gal4-driven RNAi in distinct peptidergic neurons look promising to find deficiency phenotypes.

Place, publisher, year, edition, pages
2009. Vol. 153A, no 2, suppl. 1, S79- p.
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URN: urn:nbn:se:su:diva-34719DOI: http://dx.doi.org/10.1016/j.cbpa.2009.04.053OAI: oai:DiVA.org:su-34719DiVA: diva2:285368
Available from: 2010-01-11 Created: 2010-01-11 Last updated: 2017-12-12Bibliographically approved

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