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FociCounter: A freely available PC programme for quantitative and qualitative analysis of gamma-H2AX foci
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2010 (English)In: Mutation research, ISSN 0027-5107, E-ISSN 1873-135X, Vol. 696, no 1, 16-20 p.Article in journal (Refereed) Published
Abstract [en]

Gamma-H2AX foci are sensitive and specific indicator for the induction of DNA double-strand breaks (DSBs) and an immunocytochemical assay with antibodies recognizing gamma-H2AX has become the gold standard for the detection of this type of DNA lesion. Quantification of gamma-H2AX foci can be achieved by various methods such as Western blotting, flow cytometry, visual analysis and computational analysis with a fluorescence microscope. The best sensitivity is achieved by computer analysis. Since no freeware programme for the analysis of gamma-H2AX foci exists for a PC platform, the aim of our study was to develop a simple and user-friendly public-domain software. The algorithm applied in our programme allows determination of the number of foci in a single cell, a focus intensity per cell, as well as a cell intensity. Its graphical user interface is based on a GTK+ library and the whole application can be run under a variety of operating systems, including MS Windows and Linux. The programme called FociCounter is publicly available at Application of the programme was tested by analysing gamma-H2AX foci in CHO and MO59K cells irradiated in vitro with X-rays and validated by comparing the results obtained with the outcome of automated image analysis and flow cytometry.

Place, publisher, year, edition, pages
2010. Vol. 696, no 1, 16-20 p.
Keyword [en]
Gamma-H2AX foci; Ionising radiation; Fluorescence microscopy; Automated image-analysis system; Flow cytometry
National Category
Natural Sciences
URN: urn:nbn:se:su:diva-34768DOI: 10.1016/j.mrgentox.2009.12.004ISI: 000275140600003PubMedID: 20018253OAI: diva2:285510
authorCount :7Available from: 2010-01-12 Created: 2010-01-12 Last updated: 2011-01-05Bibliographically approved

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Wojcik, Andrzej
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Department of Genetics, Microbiology and Toxicology
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