Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Gene Expression in Polytene Nuclei
Stockholm University, Faculty of Science, Department of Molecular Biology and Functional Genomics.
Stockholm University, Faculty of Science, Department of Molecular Biology and Functional Genomics.
2009 (English)In: Methods in Molecular Biology: The Nucleus / [ed] Ronald Hancock, Humana Press , 2009, 2, 29-53 p.Chapter in book (Other academic)
Abstract [en]

Gene expression in eukaryotic cells is a multi-step process. Many of the steps are both co-ordinated and quality controlled. For example, transcription is closely coupled to pre-messenger RNA (mRNA)-protein assembly, pre-mRNA processing, surveillance of the correct synthesis of messenger ribonucleoprotein (mRNP), and export. The coordination appears to be exerted through dynamic interactions between components of the transcription, processing, surveillance, and export machineries. Our knowledge is so far incomplete about these molecular interactions and where in the nucleus they take place. It is therefore essential to analyze the intranuclear steps of gene expression in vivo. Polytene nuclei are exceptionally large and contain chromosomes and individual genes that can be structurally analyzed in situ during ongoing transcription. Furthermore, they contain gene-specific pre-mRNPs/mRNPs that can be visualised and analyzed as they are synthesised on the gene and then followed on their path to the cytoplasm. We describe methods for investigating the structure and composition of active chromatin and gene-specific pre-mRNPs/mRNPs in the context of analyses of gene expression processes in the nuclei of polytene cells.

Place, publisher, year, edition, pages
Humana Press , 2009, 2. 29-53 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 464
Keyword [en]
Polytene chromosomes, Balbiani ring genes, Chromatin, Gene expression, transcription, mRNA processing, Nucleocytoplasmic export, Immunolabelling, Electron microscopy
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-35073DOI: 10.1007/978-1-60327-461-6_3ISBN: 978-1-60327-460-9 (print)OAI: oai:DiVA.org:su-35073DiVA: diva2:286318
Available from: 2010-01-14 Created: 2010-01-14 Last updated: 2011-02-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Björk, PetraWieslander, Lars
By organisation
Department of Molecular Biology and Functional Genomics
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
isbn
urn-nbn

Altmetric score

doi
isbn
urn-nbn
Total: 113 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf