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Exclusion of mRNPs and ribosomal particels from a thin zone beneath the nuclear envelope revealed upon inhibition of transport
Stockholm University, Faculty of Science, Department of Molecular Biology and Functional Genomics.
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2010 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 316, no 6, 1028-1038 p.Article in journal (Refereed) Published
Abstract [en]

We have studied the nucleocytoplasmic transport of a specific messenger RNP (mRNP) particle, named Balbiani ring (BR) granule, and ribosomal RNP (rRNP) particles in the salivary glands of the dipteran Chironomus tentans. The passage of the RNPs through the nuclear pore complex (NPC) was inhibited with the nucleoporin-binding wheat germ agglutinin, and the effects were examined by electron microscopy. BR mRNPs bound to the nuclear basket increased in number, while BR mRNPs translocating through the central channel decreased, suggesting that the initiation of translocation proper had been inhibited. The rRNPs accumulated heavily in nucleoplasm, while no or very few rRNPs were recorded within nuclear baskets. Thus, the transport of rRNPs had been blocked prior to the entry into the baskets. Remarkably, the rRNPs had been excluded both from baskets and the space in between the baskets. We propose that normally basket fibrils move freely and repel RNPs from the exclusion zone unless the particles have affinity for and bind to nucleoporins within the baskets.

Place, publisher, year, edition, pages
Elsevier , 2010. Vol. 316, no 6, 1028-1038 p.
Keyword [en]
Nucleocytoplasmic transport; Nuclear pore complex; Nuclear basket; Messenger RNA export; Messenger RNP particles; Ribosomal RNA export; Pre-ribosomal particles
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:su:diva-35363DOI: 10.1016/j.yexcr.2009.10.016ISI: 000275986000014OAI: oai:DiVA.org:su-35363DiVA: diva2:287174
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authorCount :6Available from: 2010-01-18 Created: 2010-01-18 Last updated: 2017-12-12Bibliographically approved

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