Change search
ReferencesLink to record
Permanent link

Direct link
Successful DNA amplification of a more than 200-year-old herbarium specimen: recovering genetic material from the Linnaean era
Stockholm University, Faculty of Science, Department of Botany. Stockholm University, Faculty of Science, The Bergius Botanical Garden Museum. (Bergius Foundation)
2009 (English)In: Taxon, ISSN 0040-0262, Vol. 58, no 3, 959-962 p.Article in journal (Refereed) Published
Abstract [en]

The limit for successful DNA extraction was tested by amplification and sequencing of an over 200-year-old herbarium specimen collected by Adam Afzelius, a student of Carl Linnaeus. We amplified and sequenced a 800-bp region between 16S ribosomal DNA and the 3′ part of the trnI gene (16S-trnI) in the chloroplast genomeof Phaulopsis talbotii S. Moore (Acanthaceae). To test the replicability and to control for contamination the procedure was performed in sealed vials and with negative PCR controls. The procedure was also repeated in a separate laboratory. In addition, the chloroplast rpl16 intron was successfully amplified and sequenced and the rps16 intron amplified. Sequences of taxa closely related to Acanthaceae were found to be most similar to the produced sequences. The results suggest that molecular investigations of other 18th century botanical collections are feasible and that molecular methods could be employed for comparative studies to extant plant collections. An important application would be to identify descendants or clones of Linnaean lectotypes by comparing DNA from these with potentially remnant plants from Linnaeus’ cultivations.

Place, publisher, year, edition, pages
2009. Vol. 58, no 3, 959-962 p.
Keyword [en]
16S-trnI, chloroplast DNA, DNA amplification, herbarium specimens, Phaulopsis, rpl16
National Category
Natural Sciences
URN: urn:nbn:se:su:diva-36173ISI: 000269774900023OAI: diva2:288914
Available from: 2010-01-22 Created: 2010-01-22 Last updated: 2010-11-18Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Razafimandimbison, Sylvain
By organisation
Department of BotanyThe Bergius Botanical Garden Museum
In the same journal
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 66 hits
ReferencesLink to record
Permanent link

Direct link