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Molecular phylogenetics of Alchemilla, Aphanes and Lachemilla (Rosaceae) inferred from plastid and nuclear intron and spacer DNA sequences, with comments on generic classification
University of Zurich, Institute for Systematic Botany.
Department Biologie I, Systematische Botanik, LMU Munich.
Herbario QCA, Pontificia Universidad Católica del Ecuador.
Stockholm University, Faculty of Science, Department of Botany.
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2008 (English)In: Molecular Phylogenetics and Evolution, ISSN 1055-7903, E-ISSN 1095-9513, Vol. 47, 1030-1044 p.Article in journal (Refereed) Published
Abstract [en]

Alchemilla (the lady’s mantles) is a well known but inconspicuous group in the Rosaceae, notable for its ornamental leaves and pharmaceutical properties. The systematics of Alchemilla has remained poorly understood, most likely due to confusion resulting from apomixis, polyploidisation and hybridisation, which are frequently observed in the group, and which have led to the description of a large number of (micro-) species. A molecular phylogeny of the genus, including all sections of Alchemilla and Lachemilla as well as five representatives of Aphanes, based on the analysis of the chloroplast trnL–trnF and the nuclear ITS regions is presented here. Gene phylogenies reconstructed from the nuclear and chloroplast sequence data were largely congruent. Limited conflict between the data partitions was observed with respect to a small number of taxa. This is likely to be the result of hybridisation/introgression or incomplete lineage sorting. Four distinct clades were resolved, corresponding to major geographical division and life forms: Eurasian Alchemilla, annual Aphanes, South American Lachemilla and African Alchemilla. We argue for a wider circumscription of the genus Alchemilla, including Lachemilla and Aphanes, based on the morphology and the phylogenetic relationships between the different clades.

Place, publisher, year, edition, pages
2008. Vol. 47, 1030-1044 p.
Keyword [en]
Alchemilla, Lachemilla, Aphanes, Alchemillinae, Rosaceae, Molecular phylogeny, Generic delimitation, Apomixis
National Category
Biological Sciences Biological Systematics
Research subject
Plant Systematics
URN: urn:nbn:se:su:diva-36223DOI: 10.1016/j.ympev.2008.03.004ISI: 000257128900013OAI: diva2:289032
Available from: 2010-01-22 Created: 2010-01-22 Last updated: 2011-04-26Bibliographically approved
In thesis
1. Systematics and polyploid evolution in Potentilleae (Rosaceae)
Open this publication in new window or tab >>Systematics and polyploid evolution in Potentilleae (Rosaceae)
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis comprises studies of the phylogenetic relationships in the flowering plant clade Potentilleae in Rosaceae. The relationships were elucidated by using DNA sequence data from the nuclear genome as well as from the plastid genome. In particular, the focus of the studies was the investigation of allopolyploidy, i.e. speciation as a result of hybridization and subsequent chromosome doubling. A phylogenetic method was used for identifying allopolyploidy through comparison of trees resulting from the analyses of different DNA sequences. Five sub-clades were investigated. First, both the sister clades that together contain all of Potentilleae: Fragariinae and Potentilla. Secondly, three subclades of Fragariinae, namely Alchemilla in wide sense, Sibbaldia and relatives, and Fragaria. The aim was to unravel the phylogenetic relationships, including instances of allopolyploidy. Classification issues were discussed in relation to the phylogenetic results. The split between Potentilla (=Potentillinae) and Fragariinae received better support than in previous studies. The phylogeny of Fragariinae was found to be consistent with classifying ten genera: Alchemilla in wide sense (incl. Aphanes and Lachemilla), Comarum, Sibbaldia, Sibbaldianthe, Sibbaldiopsis, Chamaerhodos, Drymocallis, Dasiphora, Potaninia, Fragaria, and also including a few orphan Potentilla species. The segregated genera Ivesia, Horkelia, Horkeliella and Duchesnea were found to be nested within Potentilla, corroborating earlier studies, while the segregated genus Argentina (P. anserina and close relatives) showed an ambiguous position. Plastid and nuclear (ribosomal) phylogenies were compared and incongruences were detected as potential instances of allopolyploid speciation. Five strongly supported incongruences were detected in Fragariinae and four of them were considered to be potentially caused by allopolyploidy. In addition, five supported incongruences were found in Potentilla. Alchemilla in the wide sense was found to contain four major clades, African Alchemilla, Eurasian Alchemilla, Lachemilla and Aphanes. Both Lachemilla and Aphanes were nested within Alchemilla and it was suggested that the name Alchemilla should be used in the wide sense, i.e. including both the genera Lachemilla and Aphanes. The genus Sibbaldia as commonly classified was shown to be polyphyletic in five different places in Potentilleae. Three Sibbaldia clades ended up in Fragariinae and two in Potentilla. A phylogeny of Fragaria, based on a nuclear low/single copy DNA region was estimated. The gene copy phylogeny was used to construct a reticulate tree hypothesizing allopolyploid speciation events. The evolution of Fragaria was shown to have been shaped by polyploidy.

Place, publisher, year, edition, pages
Stockholm: Department of Botany, Stockholm University, 2011. 28 p.
Potentilleae, Fragariinae, Potentilla, Sibbaldia, Fragaria, Alchemilla, systematics, phylogeny, polyploidy, autopolyploidy, allopolyploidy, reticulate evolution
National Category
Biological Systematics
Research subject
Plant Systematics
urn:nbn:se:su:diva-53967 (URN)978-91-7447-227-1 (ISBN)
Public defence
2011-05-20, föreläsningssalen, Botanicum, Lilla Frescativägen 5, Stockholm, 10:00 (English)
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 2: Manuscript. Paper 4: Manuscript. Paper 5: Manuscript.Available from: 2011-04-28 Created: 2011-01-25 Last updated: 2011-04-26Bibliographically approved

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Lundberg, MagnusEriksson, Torsten
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Department of BotanyThe Bergius Botanical Garden Museum
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