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Study of mass transfer in a dynamic hollow-fibreliquid phase microextraction system
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
2010 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 33, no 1, 112-119 p.Article in journal (Refereed) Published
Abstract [en]

The extraction characteristics of a dynamic hollow-fibre liquid phase microextractionsystem were investigated by studying the mass transfer and diffusion rates of dinitrophenolsfrom plasma samples over the liquid membrane (dihexylether). The measureddiffusion coefficients were compared with theoretical values calculated from Stokesdiameters. The diffusion mechanism was simulated by computer and the most polarcompounds, 2,4-dinitrophenol and 4,6-o-dinitrocresol, had associated diffusion coefficientsthat were close to the calculated theoretical values. 2-sec-Butyl-4,6 dinitrophenoland 2-tert-butyl-4,6-dinitrophenol, the compounds with the highest log P values, wereretained by the polypropylene membrane, which reduced the experimentally observeddiffusion rates to about half of the theoretical values. The retention was most likely due todispersive forces interacting with the pore inner walls. Extraction was linearly correlatedwith time for all compounds and the repeatability was high (RSDs 7–11%), even for theshortest extraction times. Method LOD as the amount injected ranged between 0.3 and3.1 ng for an extraction cycle of 213 s.

Place, publisher, year, edition, pages
Wiley Interscience , 2010. Vol. 33, no 1, 112-119 p.
Keyword [en]
dynamic hollowfibre liquid phase microextraction
National Category
Other Basic Medicine
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-38143DOI: 10.1002/jssc.200900539ISI: 000274306800014OAI: oai:DiVA.org:su-38143DiVA: diva2:306255
Available from: 2010-03-30 Created: 2010-03-29 Last updated: 2017-12-12Bibliographically approved
In thesis
1. A Novel Miniaturised Dynamic Hollow-Fibre Liquid-Phase Micro-Extraction Method for Xenobiotics in Human Plasma Samples
Open this publication in new window or tab >>A Novel Miniaturised Dynamic Hollow-Fibre Liquid-Phase Micro-Extraction Method for Xenobiotics in Human Plasma Samples
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bioanalytical chemistry is a challenging field, often involving complex samples, such as blood, plasma, serum or urine. In many applications, sample cleanup is the most demanding and time-consuming step.

In the work underlying this thesis a novel dynamic miniature extractor, known as a hollow-fibre liquid-phase microextractor (HF-LPME), was designed, evaluated and studied closely when used to clean plasma samples. Aqueous-organic-aqueous liquid extraction, in which the organic liquid is immobilised in a porous polypropylene membrane, was the principle upon which the extractor was based, and this is discussed in all the papers associated with this thesis. This type of extraction is known as supported-liquid membrane extraction (SLM). The aim of this work was the development of a dynamic system for SLM. It was essential that the system could handle small sample volumes and had the potential for hyphenations and on-line connections to, for instance, LC/electrospray-MS. The design of a miniaturised HF-LPME device is presented in Paper I. The extraction method was developed for some weakly acidic pesticides and these were also used for evaluation. In the work described in Paper II, the method was optimised on the basis of an experimental design using spiked human plasma samples. Paper III presents a detailed study of the mass-transfer over the liquid membrane. The diffusion through the membrane pores was illustrated by a computer-simulation. Not surprisingly, the more lipophilic, the greater the retention of the compounds, as a result of dispersive forces. The main focus of the work described in Paper IV was to make the HF/LPME system more versatile and user-friendly; therefore, the extractor was automated by hyphenation to a SIA system.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2010. 97 p.
Keyword
sample clean-up, human blood plasma, hollow-fibre liquid phase microextraction, supported liquid membrane extraction, SLM, HF-LPME, sample preparation, bioanalysis, liquid-liquid extraction
National Category
Other Basic Medicine
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-41742 (URN)978-91-7447-128-1 (ISBN)
Public defence
2010-09-29, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 10:00 (Swedish)
Opponent
Supervisors
Note
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 4: Manuscript. Available from: 2010-09-07 Created: 2010-08-05 Last updated: 2011-05-02Bibliographically approved

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