Assessment of a dynamic hollow-fibre liquid phase microextraction system forhuman blood plasma samples
2009 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 77, no 4, 1309-1314 p.Article in journal (Refereed) Published
A dynamic liquid phase microextraction (LPME) system, based on hollow-fibre supported liquid membrane(SLM) extraction, was developed for extracting ionisable xenobiotics from human plasma, andits performance was evaluated (in terms of extraction efficiency, reproducibility, durability and carryover)using nitrophenolic compounds as model analytes at concentrations of 0.1–0.5gmL−1 in aqueousstandards. The efficiency and repeatability were tested also on spiked human plasma. The system isnon-expensive, convenient, requires minimal manual handling and enables samples with volumes assmall as 0.2mL to be extracted. For plasma samples extraction efficiencies of between 30 and 58% wereachieved within 20 min, including washing steps. The limit of detection (LOD) values were in the range0.02–0.03gmL−1. The developed system can provide enrichment factors up to eight, based on theinjection-to-acceptor volume ratio (in this case 0.2–0.025 mL). The same hollow-fibre membrane wasused up to 8 days with no loss of efficiency. Carry-over was lower than detection limit.
Place, publisher, year, edition, pages
Elsevier , 2009. Vol. 77, no 4, 1309-1314 p.
Supported liquid membrane
Other Basic Medicine
Research subject Analytical Chemistry
IdentifiersURN: urn:nbn:se:su:diva-38145DOI: 10.1016/j.talanta.2008.09.005ISI: 000262761500009OAI: oai:DiVA.org:su-38145DiVA: diva2:306259