Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Assessment of a dynamic hollow-fibre liquid phase microextraction system forhuman blood plasma samples
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
2009 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 77, no 4, 1309-1314 p.Article in journal (Refereed) Published
Abstract [en]

A dynamic liquid phase microextraction (LPME) system, based on hollow-fibre supported liquid membrane(SLM) extraction, was developed for extracting ionisable xenobiotics from human plasma, andits performance was evaluated (in terms of extraction efficiency, reproducibility, durability and carryover)using nitrophenolic compounds as model analytes at concentrations of 0.1–0.5gmL−1 in aqueousstandards. The efficiency and repeatability were tested also on spiked human plasma. The system isnon-expensive, convenient, requires minimal manual handling and enables samples with volumes assmall as 0.2mL to be extracted. For plasma samples extraction efficiencies of between 30 and 58% wereachieved within 20 min, including washing steps. The limit of detection (LOD) values were in the range0.02–0.03gmL−1. The developed system can provide enrichment factors up to eight, based on theinjection-to-acceptor volume ratio (in this case 0.2–0.025 mL). The same hollow-fibre membrane wasused up to 8 days with no loss of efficiency. Carry-over was lower than detection limit.

Place, publisher, year, edition, pages
Elsevier , 2009. Vol. 77, no 4, 1309-1314 p.
Keyword [en]
Supported liquid membrane
National Category
Other Basic Medicine
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-38145DOI: 10.1016/j.talanta.2008.09.005ISI: 000262761500009OAI: oai:DiVA.org:su-38145DiVA: diva2:306259
Available from: 2010-03-30 Created: 2010-03-29 Last updated: 2017-12-12Bibliographically approved
In thesis
1. A Novel Miniaturised Dynamic Hollow-Fibre Liquid-Phase Micro-Extraction Method for Xenobiotics in Human Plasma Samples
Open this publication in new window or tab >>A Novel Miniaturised Dynamic Hollow-Fibre Liquid-Phase Micro-Extraction Method for Xenobiotics in Human Plasma Samples
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bioanalytical chemistry is a challenging field, often involving complex samples, such as blood, plasma, serum or urine. In many applications, sample cleanup is the most demanding and time-consuming step.

In the work underlying this thesis a novel dynamic miniature extractor, known as a hollow-fibre liquid-phase microextractor (HF-LPME), was designed, evaluated and studied closely when used to clean plasma samples. Aqueous-organic-aqueous liquid extraction, in which the organic liquid is immobilised in a porous polypropylene membrane, was the principle upon which the extractor was based, and this is discussed in all the papers associated with this thesis. This type of extraction is known as supported-liquid membrane extraction (SLM). The aim of this work was the development of a dynamic system for SLM. It was essential that the system could handle small sample volumes and had the potential for hyphenations and on-line connections to, for instance, LC/electrospray-MS. The design of a miniaturised HF-LPME device is presented in Paper I. The extraction method was developed for some weakly acidic pesticides and these were also used for evaluation. In the work described in Paper II, the method was optimised on the basis of an experimental design using spiked human plasma samples. Paper III presents a detailed study of the mass-transfer over the liquid membrane. The diffusion through the membrane pores was illustrated by a computer-simulation. Not surprisingly, the more lipophilic, the greater the retention of the compounds, as a result of dispersive forces. The main focus of the work described in Paper IV was to make the HF/LPME system more versatile and user-friendly; therefore, the extractor was automated by hyphenation to a SIA system.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2010. 97 p.
Keyword
sample clean-up, human blood plasma, hollow-fibre liquid phase microextraction, supported liquid membrane extraction, SLM, HF-LPME, sample preparation, bioanalysis, liquid-liquid extraction
National Category
Other Basic Medicine
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-41742 (URN)978-91-7447-128-1 (ISBN)
Public defence
2010-09-29, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 10:00 (Swedish)
Opponent
Supervisors
Note
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 4: Manuscript. Available from: 2010-09-07 Created: 2010-08-05 Last updated: 2011-05-02Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Hansson, HelenaNilsson, Ulrika
By organisation
Department of Analytical Chemistry
In the same journal
Talanta: The International Journal of Pure and Applied Analytical Chemistry
Other Basic Medicine

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 40 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf