SALDI-MS Signal Enhancement Using Oxidized Graphitized Carbon Black Nanoparticles
2009 (English)In: Journal of the American Society for Mass Spectrometry, ISSN 1044-0305, E-ISSN 1879-1123, Vol. 20, no 6, 1207-1213 p.Article in journal (Refereed) Published
The signal intensity of low-molecular-weight compounds analyzed using surface-assisted laserdesorption/ionization time-of-flight mass spectrometry (SALDI-TOF-MS) was significantlyenhanced when oxidized graphitized carbon black (GCB) particles were used as the desorption/ionization surface. The surface of oxidized GCB contains more carboxylic acid groupsthan non-oxidized GCB. Carboxylic acid groups enhance the efficiency of the ionizationprocess and the desorption of more hydrophobic compounds. A common pharmaceuticalcompound, propranolol, was successfully extracted from Baltic Sea blue mussels and quantifiedusing oxidized GCB as the SALDI surface, whereas deuterated propranolol was used asthe internal standard. The calibration curve showed a wide linear dynamic range of response(0.1–20 g/mL) and good reproducibility (RSD 10%). It was not possible to detectpropranolol in Baltic Sea blue mussels when non-oxidized GCB was used as the SALDI surface.
Place, publisher, year, edition, pages
2009. Vol. 20, no 6, 1207-1213 p.
IdentifiersURN: urn:nbn:se:su:diva-38262DOI: 10.1016/j.jasms.2009.02.017ISI: 000266466600036OAI: oai:DiVA.org:su-38262DiVA: diva2:308472