Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Silicon nitride nanoparticles for surface-assisted laser desorption/ionization of small molecules
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
2009 (English)In: Journal of nanoparticle research, ISSN 1388-0764, E-ISSN 1572-896X, Vol. 11, no 6, 1509-1512 p.Article in journal (Refereed) Published
Abstract [en]

Conventional matrix-assisted laser desorption/ionization mass spectrometry is limited to analyses of higher molecular weight compounds due to high background noise generated by the matrix in the lower mass region. Surface-assisted laser desorption/ionization (SALDI) mass spectrometry is an alternative solution to this problem. Nanoparticles, structured silicon surfaces and carbon allotropes are commonly used as SALDI surfaces. Here, for the first time, we demonstrate the application of silicon nitride nanoparticles as a suitable medium for laser desorption/ionization of small drug molecules.

Place, publisher, year, edition, pages
2009. Vol. 11, no 6, 1509-1512 p.
Identifiers
URN: urn:nbn:se:su:diva-38263DOI: 10.1007/s11051-009-9601-6OAI: oai:DiVA.org:su-38263DiVA: diva2:308479
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Novel Solid Phase Extraction and Mass Spectrometry Approaches to Multicomponent Analyses in Complex Matrices
Open this publication in new window or tab >>Novel Solid Phase Extraction and Mass Spectrometry Approaches to Multicomponent Analyses in Complex Matrices
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Analysis of compounds present in complex matrices is always a challenge, which can be partly overcome by applying various sample preparation techniques prior to detection. Ideally, the extraction techniques should be as selective as possible, to minimize the concentration of interfering substances. In addition, results can be improved by efficient chromatographic separation of the sample components. The elimination of interfering substances is especially important when utilizing mass spectrometry (MS) as a detection technique since they influence the ionization yields. It is also important to optimize ionization methods in order to minimize detection limits.

In the work this thesis is based upon, selective solid phase extraction (SPE) materials, a restricted access material (RAM) and graphitized carbon black (GCB) were employed for clean up and/or pre-concentration of analytes in plasma, urine and agricultural drainage water prior to liquid chromatography/mass spectrometry (LC/MS). Two SPE formats, in which GCB was incorporated in µ-traps and disks, were developed for cleaning up small and large volume samples, respectively. In addition, techniques based on use of sub-2 µm C18 particles at elevated temperatures and a linear ion trap (LIT) mass spectrometer were developed to improve the efficiency of LC separation and sensitivity of detection of 6-formylindolo[3,2-b]carbazole (FICZ) metabolites in human urine.

It was also found that GCB can serve not only as a SPE sorbent, but also as a valuable surface for surface-assisted laser desorption ionization (SALDI) of small molecules. The dual functionality of GCB was utilized in a combined screening-identification/quantification procedure for fast elimination of negative samples. This may be particularly useful when processing large numbers of samples. SALDI analyses of small molecules was further investigated and improved by employing two kinds of new surfaces: oxidized GCB nanoparticles and silicon nitride.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2010. 62 p.
Keyword
Sample preparation, SPE, RAM, GCB µ-trap, GCB disk, High temperature LC, LC/MS, ESI, APCI, Matrix effects, LIT-MS, SALDI, Sample screening, Oxidized GCB, Silicon nitride
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-38625 (URN)978-91-7447-050-5 (ISBN)
Public defence
2010-06-02, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 10:00 (English)
Opponent
Supervisors
Available from: 2010-05-11 Created: 2010-04-22 Last updated: 2010-05-04Bibliographically approved
2. Novel clean-up, concentration and laser desorption/ionization strategies for mass spectrometry
Open this publication in new window or tab >>Novel clean-up, concentration and laser desorption/ionization strategies for mass spectrometry
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Despite valuable advantages of mass spectrometry (MS) techniques they still have drawbacks that need to be overcome, notably their sensitivity to various kinds of interfering substances and associated difficulties involved in detecting signals from analytes present in trace quantities. Thus, high quality analytical data could be acquired by improving sample pre-treatment techniques and/or ionization methods to increase ionization yields and/or avoid the generation of unwanted ions.

In the studies this thesis is based upon, a microfluidic electrocapture system capable of trapping charged proteins/polypeptides while non-charged species such as non-ionic detergents are swept out, was used to decrease the concentration of detergent in samples containing soluble and membrane associated proteins/polypeptide. Subsequent mass spectrometric analysis yielded well-resolved mass spectra of the target analytes.

In addition, it was found that solid phase extraction (SPE) sorbents such as graphitized carbon black (GCB), can efficiently assist the laser desorption ionization of small molecules without generating any interfering ions which inspired the fabrication of μ-traps and discs. Other matrix-free approaches demonstrated the utility of silicon nitride and oxidized GCB nanoparticles as versatile media for surface-assisted laser desorption ionization (SALDI) of small molecules.

In this thesis it is discussed that small molecule analytes such as Polyphenols present in red wine and quaternary protoberberine alkaloids with highly conjugated systems and acidic/permanently charged functional groups can be readily analyzed without any matrix or assisting-surface.

In summary, the microfluidic electrocapture, new laser desorption ionization methods and surfaces, as well as integrated SALDI and SPE platforms, introduced in this work extend the MS strategies allowing coverage of a wider range of samples.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2010. 77 p.
Keyword
Mass spectrometry, ESI-MS, SALDI-MS, Electrocapture, Sample treatment
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:su:diva-32236 (URN)978-91-7155-981-4 (ISBN)
Public defence
2010-01-22, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 10:00 (English)
Opponent
Supervisors
Note
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: In PressAvailable from: 2009-12-17 Created: 2009-12-07 Last updated: 2011-08-31Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Shariatgorji, MohammadrezaAmini, NahidIlag, Leopold L.
By organisation
Department of Analytical Chemistry
In the same journal
Journal of nanoparticle research

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 121 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf