Identification of bases required for P2 Integrase core-binding and recombination
(English)Manuscript (preprint) (Other academic)
Temperate coliphage P2 integrates its genome into the host chromosome upon lysogenization via a site-specific recombination event mediated by an integrase belonging to the complex family of tyrosine recombinases. The host integration site attB (BOB´) is localized in the end of the cyaR gene and share 27 nucleotides with the core of attP (COC´). In the present study we determine the minimal attB site using an in vivo recombination assay and 10 nt on the left side (B) are found to be nonessential for recombination. We show that the integrase has higher affinity for the right side (B´) compared to B and that artificial B´OB´ and an attP site with a matching core (C´OC´) are efficient substrates for recombination in vitro. We have analyzed single nucleotides in attB and find that sequence homology within a non-centrally located quadruplet in the hypotetical overlap region is essential for efficient recombination in vivo.
Site-specific recombination, integrase, tyrosine recombinase, bacteriophage
Genetics Cell and Molecular Biology Microbiology in the medical area
Research subject Molecular Genetics
IdentifiersURN: urn:nbn:se:su:diva-38925OAI: oai:DiVA.org:su-38925DiVA: diva2:317568