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In vivo identification of ribonucleoprotein–RNA interactions
Stockholm University, Faculty of Science, Department of Neurochemistry.
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2006 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 103, no 5, 1557-1562 p.Article in journal (Refereed) Published
Abstract [en]

To understand the role of RNA-binding proteins (RBPs) in the regulation of gene expression, methods are needed for the in vivo identification of RNA–protein interactions. We have developed the peptide nucleic acid (PNA)-assisted identification of RBP technology to enable the identification of proteins that complex with a target RNA in vivo. Specific regions of the 3ˈ and 5ˈ UTRs of ankylosis mRNA were targeted by antisense PNAs transported into cortical neurons by the cell-penetrating peptide transportan 10. An array of proteins was isolated in complex with or near the targeted regions of the ankylosis mRNA through UV-induced crosslinking of the annealed PNA–RNA–RBP complex. The first evidence for pharmacological modulation of these specific protein–RNA associations was observed. These data show that the PNA-assisted identification of the RBP technique is a reliable method to rapidly identify proteins interacting in vivo with the target RNA.

Place, publisher, year, edition, pages
2006. Vol. 103, no 5, 1557-1562 p.
Keyword [en]
RNA-binding proteins
National Category
Microbiology in the medical area
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URN: urn:nbn:se:su:diva-39089DOI: 10.1073/pnas.0510611103OAI: oai:DiVA.org:su-39089DiVA: diva2:318348
Note

Jennifer Zielinski and Kalle Kilk contributed equally to this work.

Available from: 2010-05-07 Created: 2010-05-07 Last updated: 2017-12-12Bibliographically approved

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