Identification and immunocytochemical localization of proctolin in pericardial organs of the shore crab, Carcinus maenas.
1986 (English)In: Peptides, ISSN 0196-9781, E-ISSN 1873-5169, Vol. 7, no 1, 67-72 p.Article in journal (Refereed) Published
The occurrence of proctolin (Arg-Tyr-Leu-Pro-Thr) in crab neurohemal pericardial organs (POs) has been demonstrated by isolation of the pentapeptide by HPLC and manual microsequencing according to the DABITC-PITC double coupling technique. From one pair of POs approximately 5.4 pmol were obtained (= 45 pmol/mg protein). Immunocytochemically, an extensive system of positive structures was found in both whole mount preparations and semithin sections, consisting of numerous varicose fibres of varying diameter and many knoblike neurosecretory terminals abutting upon the epineurium of the PO trunks. The relatively high concentration in the POs as well as the pattern of proctolin-positive fibres and terminals clearly suggest a neurohormonal role of the pentapeptide in decapod crustaceans.
Place, publisher, year, edition, pages
Elsevier , 1986. Vol. 7, no 1, 67-72 p.
IdentifiersURN: urn:nbn:se:su:diva-40044DOI: 10.1016/0196-9781(86)90063-XPubMedID: 2872661OAI: oai:DiVA.org:su-40044DiVA: diva2:326356