Change search
ReferencesLink to record
Permanent link

Direct link
Occurrence of analogues of the myotropic neuropeptide orcokinin in the shore crab, Carcinus maenas: evidence for a novel neuropeptide family.
Institute of Zoophysiology, University of Bonn, Germany.ORCID iD: 0000-0001-7815-4868
1995 (English)In: Peptides, ISSN 0196-9781, E-ISSN 1873-5169, Vol. 16, no 1, 67-72 p.Article in journal (Refereed) Published
Abstract [en]

By use of an enzyme immunoassay that was developed for the determination of orcokinin, a myotropic neuropeptide of the sequence NFDEIDRSGFGFN from the crayfish, Orconectes limosus, immunoreactive material was detected in extracts of thoracic ganglia from the shore crab, Carcinus maenas. Isolation of the immunoreactive material was achieved by the following steps: 1) prepurification by gel filtration, 2) immunoaffinity chromatography on an anti-orcokinin IgG protein-A sepharose column, and 3) reversed-phase HPLC. The HPLC profile after affinity purification revealed three main immunoreactive peptides that were rechromatographed. None of these peptides was identical to orcokinin in terms of retention time. Automated gas-phase sequencing revealed these peptides to be analogues of orcokinin differing in one amino acid residue. They were named [Ser9]-, [Ala13]- and [Val13]orcokinin (NFDEIDRSSFGFN, Mr 1549.3; NFDEIDRSGFGFA, Mr 1475.3; NFDEIDRSGFGFV, Mr 1503.9). Carboxypeptidase A treatment of the peptides indicated a free C-terminus. Complete characterization of the three peptides was achieved from approximately 230 thoracic ganglia of Carcinus maenas.

Place, publisher, year, edition, pages
1995. Vol. 16, no 1, 67-72 p.
National Category
Biological Sciences
URN: urn:nbn:se:su:diva-40036DOI: 10.1016/0196-9781(94)00145-VPubMedID: 7716076OAI: diva2:326363
Available from: 2010-06-22 Created: 2010-06-04 Last updated: 2014-10-13Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Dircksen, Heinrich
In the same journal
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 54 hits
ReferencesLink to record
Permanent link

Direct link