Change search
ReferencesLink to record
Permanent link

Direct link
Crustacean hyperglycaemic hormone (CHH)-like peptides and CHH-precursor-related peptides from pericardial organ neurosecretory cells in the shore crab, Carcinus maenas, are putatively spliced and modified products of multiple genes.
Institute of Zoophysiology, University of Bonn, Germany.ORCID iD: 0000-0001-7815-4868
Show others and affiliations
2001 (English)In: Biochemical Journal, ISSN 0264-6021, E-ISSN 1470-8728, Vol. 356, no Pt 1, 159-70 p.Article in journal (Refereed) Published
Abstract [en]

About 24 intrinsic neurosecretory neurons within the pericardial organs (POs) of the crab Carcinus maenas produce a novel crustacean hyperglycaemic hormone (CHH)-like peptide (PO-CHH) and two CHH-precursor-related peptides (PO-CPRP I and II) as identified immunochemically and by peptide chemistry. Edman sequencing and MS revealed PO-CHH as a 73 amino acid peptide (8630 Da) with a free C-terminus. PO-CHH and sinus gland CHH (SG-CHH) share an identical N-terminal sequence, positions 1-40, but the remaining sequence, positions 41-73 or 41-72, differs considerably. PO-CHH may have different precursors, as cDNA cloning of PO-derived mRNAs has revealed several similar forms, one exactly encoding the peptide. All PO-CHH cDNAs contain a nucleotide stretch coding for the SG-CHH(41-76) sequence in the 3'-untranslated region (UTR). Cloning of crab testis genomic DNA revealed at least four CHH genes, the structure of which suggest that PO-CHH and SG-CHH arise by alternative splicing of precursors and possibly post-transcriptional modification of PO-CHH. The genes encode four exons, separated by three variable introns, encoding part of a signal peptide (exon I), the remaining signal peptide residues, a CPRP, the PO-CHH(1-40)/SG-CHH(1-40) sequences (exon II), the remaining PO-CHH residues (exon III) and the remaining SG-CHH residues and a 3'-UTR (exon IV). Precursor and gene structures are more closely related to those encoding related insect ion-transport peptides than to penaeid shrimp CHH genes. PO-CHH neither exhibits hyperglycaemic activity in vivo, nor does it inhibit Y-organ ecdysteroid synthesis in vitro. From the morphology of the neurons it seems likely that novel functions remain to be discovered.

Place, publisher, year, edition, pages
2001. Vol. 356, no Pt 1, 159-70 p.
National Category
Biological Sciences
URN: urn:nbn:se:su:diva-40026PubMedID: 11336648OAI: diva2:326379
Available from: 2010-06-23 Created: 2010-06-04 Last updated: 2014-10-13Bibliographically approved

Open Access in DiVA

fulltext(339 kB)265 downloads
File information
File name FULLTEXT01.pdfFile size 339 kBChecksum SHA-512
Type fulltextMimetype application/pdf


Search in DiVA

By author/editor
Dircksen, Heinrich
In the same journal
Biochemical Journal
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 265 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 100 hits
ReferencesLink to record
Permanent link

Direct link