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Mobilization of metals from uranium mine waste: the role of pyoverdines produced by Pseudomonas fluorescens
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
Swedish Nuclear Fuel and Waste Mangament Co, Stockholm .
Stockholm University, Faculty of Science, Department of Geological Sciences.
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM).
2010 (English)In: Geobiology, ISSN 1472-4677, E-ISSN 1472-4669, Vol. 8, no 4, 278-292 p.Article in journal (Refereed) Published
Abstract [en]

Microorganisms produce chelating agents, such as siderophores and other ligands, which allow them to mobilize and scavenge essential elements from the environment when bioavailability is low. To better understand the effects of biologically mediated leaching of metals from mine waste, Pseudomonas fluorescens was cultivated in the presence of processed ore from the former uranium mine in Ranstad, southern Sweden. Light conditions, the concentration of the mineral source and oxygen availability were varied. The presence of ore in the culture flasks enhanced bacterial growth and raised the pH of the culture medium. Increasing the amount of ore or enhancing aeration of the medium further encouraged cell growth and pH rise. Bacteria mobilized Fe, Ni and Co from the ore. Fe-siderophore complexes were detected and estimated to be present at approximately 9 μm. In the presence of bacteria and light, dissolved Fe and U concentrations were higher compared to dark conditions. Increasing the amount of ore resulted in higher dissolved Ni concentrations but lower dissolved Fe, most likely due to precipitate formation. Data from this study support siderophore production by bacteria that allowed mobilization of essential nutrients from the processed ore. However, the availability of potentially toxic metals like Ni and U may also be enhanced. Microbial-promoted mobilization could contribute to leaching of toxic metals in current and historic mining areas. This process should be considered during design and implementation of remediation projects where trace metals are of environmental concern.

Place, publisher, year, edition, pages
2010. Vol. 8, no 4, 278-292 p.
National Category
Natural Sciences
Research subject
Applied Environmental Science
Identifiers
URN: urn:nbn:se:su:diva-43170DOI: 10.1111/j.1472-4669.2010.00241.xISI: 000280997500003OAI: oai:DiVA.org:su-43170DiVA: diva2:354423
Available from: 2010-10-01 Created: 2010-10-01 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Bacteria and geochemistry in a former uranium open pit mine - mobilization of trace metals
Open this publication in new window or tab >>Bacteria and geochemistry in a former uranium open pit mine - mobilization of trace metals
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In mining areas around the world, chemical and biotic processes have lead to the release of toxic substances, e.g. metals. Microbial processes influence metal speciation, while the chemical environment shapes and controls microbial populations. This thesis has two parts and the papers cover various aspects of metal-to-microbe links in a former uranium mine at Ranstad, Sweden.

The first part of the thesis examines bacteria-induced metal mobilization by the cultivation of Pseudomonas fluorescens with ore and minerals from the Ranstad mine. The presence of a mineral source stimulated bacterial growth. Pyoverdine-type siderophores typical for P. fluorescens were produced, but also other types of siderophores. The bacterial mobilization of metals is implied by the agreement in concentrations of Fe-pyoverdine complex and soluble Fe, and by the concentrations of Ni and Co in solution. U was mobilized from the ore as a result of bacterial growth raising pH.

The second part of the thesis focus on the partitioning of metals and their links to the microbial communities in the water of the former open pit mine, Lake Tranebärssjön. The lake was pH-neutral to alkaline, highly stratified, and had an anoxic hypolimnion with high concentrations of metals and SO42-. Size fractionation showed the dissolved fraction dominating for most metals, while the particulate and colloidal fractions dominated for Fe. Chemical equilibrium models agreed reasonably well with these results. In the pyrosequencing study of the bacterial community, depth-related changes in water chemistry corresponded to a distinct shift in the microbial community, indicating a chemical control. The presence of metal- and SO42--reducing bacteria suggests a possible microbe – chemistry connection.

The findings of this thesis could be used for the restoration and remediation of mining sites, and provides information on factors governing the establishment and control of bacterial populations in freshwaters.

Place, publisher, year, edition, pages
Stockholm: Department of Applied Environmental Science (ITM), Stockholm University, 2011. 48 p.
Keyword
Pseudomonas fluorescens, siderophore, Ranstad, alum shale, iron, pyrosequencing, redox, size fractionation
National Category
Natural Sciences
Research subject
Applied Environmental Science
Identifiers
urn:nbn:se:su:diva-55111 (URN)978-91-7447-241-7 (ISBN)
Public defence
2011-04-08, De Geersalen, Geovetenskapens hus, Svante Arrhenius väg 14, Stockholm, 13:00 (English)
Opponent
Supervisors
Note
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: Manuscript. Paper 4: Manuscript. Available from: 2011-03-17 Created: 2011-03-01 Last updated: 2011-04-15Bibliographically approved

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