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Novel fatty acid modifications of transportan 10
Stockholm University, Faculty of Science, Department of Neurochemistry. University of Tartu, Estonia.
Stockholm University, Faculty of Science, Department of Neurochemistry.ORCID iD: 0000-0001-7746-8574
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2010 (English)In: International Journal of Peptide Research and Therapeutics, ISSN 1573-3149, Vol. 16, no 4, 247-255 p.Article in journal (Refereed) Published
Abstract [en]

Cell-penetrating peptides (CPPs) are able toefficiently internalize into cells and can therefore be usedas vectors for non-viral cellular delivery of different cargoes.Previous studies have shown that hydrophobicmodifications of different CPPs can increase their transfectionefficiency dramatically. In this study we havemodified the cell penetrating-peptide transportan 10 (TP10)with a variety of hydrophobic molecules to determine therole of hydrophobicity in the uptake of these molecules.The results can be used to synthesize more efficientdelivery vectors. To evaluate how these constructs are ableto transport cargoes into cells we used 20-OMe splicecorrecting oligonucleotides. Non-covalent peptide-cargocomplexes were formed and their transfection efficiencywas measured using a luciferase readout system. Thehydrophobicity of the novel modifications was correlatedwith their biological efficacy. We determined the mostefficient range of hydrophobicity for TP10 analogs fordelivering oligonucleotides into cells. In order to assesshow the transfection efficacy of these particles is dependenton their size the hydrodynamic diameter of the formednanoparticles was measured using dynamic light scattering.These findings will be used to develop highly efficient nonviralgene therapy vectors

Place, publisher, year, edition, pages
2010. Vol. 16, no 4, 247-255 p.
Keyword [en]
Cell-penetrating peptide, Gene delivery, Oligonucleotide delivery, Splice correction, Dynamic light scattering
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-43393DOI: 10.1007/s10989-010-9224-xISI: 000283948500005OAI: oai:DiVA.org:su-43393DiVA: diva2:356186
Available from: 2010-10-11 Created: 2010-10-11 Last updated: 2015-04-21Bibliographically approved

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Lindberg, StaffanSillard, RannarLangel, Ülo
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