Following Enzyme Activity with Infrared Spectroscopy
2010 (English)In: Sensors, ISSN 1424-8220, Vol. 10, no 4, 2626-2637 p.Article in journal (Refereed) Published
Fourier transform infrared (FTIR) spectroscopy provides a direct, ""on-line"" monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given using the enzymes pyruvate kinase, fumarase and alcohol dehydrogenase. The main advantage of the infrared method is that it observes the reaction of interest directly, i.e., no activity assay is required to convert the progress of the reaction into an observable quantity.
Place, publisher, year, edition, pages
2010. Vol. 10, no 4, 2626-2637 p.
vibrational spectroscopy, infrared spectroscopy, ATR, FTIR, enzyme activity
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-49235DOI: 10.3390/s100402626ISI: 000277159700008OAI: oai:DiVA.org:su-49235DiVA: diva2:376754
authorCount :22010-12-132010-12-132010-12-13Bibliographically approved