Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Neurite degeneration in differentiated human neuroblastoma cells.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.ORCID iD: 0000-0001-6298-201X
Stockholm University, Faculty of Science, Department of Neurochemistry.
Show others and affiliations
1998 (English)In: Toxicology in Vitro, ISSN 0887-2333, E-ISSN 1879-3177, Vol. 12, no 5, 557-60 p.Article in journal (Refereed) Published
Abstract [en]

We have studied neurite degeneration in differentiated human neuroblastoma (SH-SY5Y) cells. The axonopathy-inducing potency in vitro of caffeine, diazepam, methylmercury chloride (MeHg), triethyltin chloride (TET) and acrylamide (ACR) was elucidated. After 72 hours of exposure the neurite degeneration was determined (by morphological quantification) as well as the total protein content (general cytotoxicity). The concentrations that caused 20% reduction of number of neurites (ND(20)) for ACR (250+/-36 mum) and TET (0.097+/-0.03 mum) was significantly lower, 63% and 35%, respectively (P</=0.005), as compared to corresponding inhibition of general cytotoxicity (IC(20)). The effects of TET on the neurites may be related to the disturbance in Ca(2+)-signalling, and thus a secondary event. The ND(20)s for caffeine and diazepam, which are compounds without a known neurite degenerative potency in vivo, were higher as compared with the IC(20). For MeHg which is an extremely cyto- and neurotoxic compound the ND(20) was not statistically different from the IC(20), indicating that degeneration of the neurites is not a primary effect. This study indicates that the SH-SY5Y-neurite degeneration model is useful for the identification of axonopathy-inducing substances.

Place, publisher, year, edition, pages
1998. Vol. 12, no 5, 557-60 p.
Identifiers
URN: urn:nbn:se:su:diva-50344PubMedID: 20654440OAI: oai:DiVA.org:su-50344DiVA: diva2:380857
Available from: 2010-12-22 Created: 2010-12-22 Last updated: 2017-12-11Bibliographically approved

Open Access in DiVA

No full text

PubMed

Search in DiVA

By author/editor
Forsby, A
By organisation
Department of Neurochemistry
In the same journal
Toxicology in Vitro

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 659 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf