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Surface-coupled proton exchange of a membrane-bound proton acceptor
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
2010 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 107, no 9, 4129-4134 p.Article in journal (Refereed) Published
Abstract [en]

Proton-transfer reactions across and at the surface of biological membranes are central for maintaining the transmembrane proton electrochemical gradients involved in cellular energy conversion. In this study, fluorescence correlation spectroscopy was used to measure the local protonation and deprotonation rates of single pH-sensitive fluorophores conjugated to liposome membranes, and the dependence of these rates on lipid composition and ion concentration. Measurements of proton exchange rates over a wide proton concentration range, using two different pH-sensitive fluorophores with different pK(a)s, revealed two distinct proton exchange regimes. At high pH (>8), proton association increases rapidly with increasing proton concentrations, presumably because the whole membrane acts as a proton-collecting antenna for the fluorophore. In contrast, at low pH (<7), the increase in the proton association rate is slower and comparable to that of direct protonation of the fluorophore from the bulk solution. In the latter case, the proton exchange rates of the two fluorophores are indistinguishable, indicating that their protonation rates are determined by the local membrane environment. Measurements on membranes of different surface charge and at different ion concentrations made it possible to determine surface potentials, as well as the distance between the surface and the fluorophore. The results from this study define the conditions under which biological membranes can act as proton-collecting antennae and provide fundamental information on the relation between the membrane surface charge density and the local proton exchange kinetics.

Place, publisher, year, edition, pages
2010. Vol. 107, no 9, 4129-4134 p.
Keyword [en]
biomembrane, diffusion, electrostatic potential, fluorescence correlation spectroscopy (FCS), proton transfer
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:su:diva-50053DOI: 10.1073/pnas.0908671107ISI: 000275131100035OAI: oai:DiVA.org:su-50053DiVA: diva2:382757
Note
authorCount :4Available from: 2011-01-03 Created: 2010-12-21 Last updated: 2017-12-11Bibliographically approved

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Salomonsson, LinaBrzezinski, Peter
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