4-Nitrobenzoate inhibits coenzyme Q biosynthesis in mammalian cell cultures
2010 (English)In: Nature chemical biology, ISSN 1552-4450, Vol. 6, no 7, 515-517 p.Article in journal (Refereed) Published
Coenzyme Q (Q) is an electron transporter in the respiratory chain and a lipid-soluble antioxidant that decreases in humans with age. Here we show that 4-nitrobenzoate inhibited 4-hydroxybenzoate: polyprenyl transferase (Coq2) in a competitive manner and dose-dependently decreased Q in mammalian cells without accumulation of Q intermediates. As 4-nitrobenzoate neither interfered with mitochondrial respiration nor induced oxidative stress, it should prove a valuable tool for studies on both Q deficiency and Q supplementation.
Place, publisher, year, edition, pages
2010. Vol. 6, no 7, 515-517 p.
IdentifiersURN: urn:nbn:se:su:diva-50654DOI: 10.1038/NCHEMBIO.372ISI: 000278878700011OAI: oai:DiVA.org:su-50654DiVA: diva2:383219
authorCount :42011-01-042010-12-302011-01-04Bibliographically approved