Change search
ReferencesLink to record
Permanent link

Direct link
Detection of the bloom-forming cold-water dinoflagellate Biecheleria baltica in the Baltic Sea using LSU rRNA probes
Stockholm University, Faculty of Science, Department of Systems Ecology.
Show others and affiliations
2010 (English)In: Aquatic Microbial Ecology, ISSN 0948-3055, E-ISSN 1616-1564, Vol. 61, no 2, 129-140 p.Article in journal (Refereed) Published
Abstract [en]

Cold-water dinoflagellates frequently dominate the spring phytoplankton community of the northern Baltic Sea and contribute substantially to the spring primary production. These dinoflagellate communities are largely composed of 3 different species (Biecheleria baltica, Scrippsiella hangoei, Gymnodinium corollarium) that cannot be unambiguously separated by conventional light microscopy. In this study, a fluorescence in situ hybridization (FISH) assay was developed for the detection of B. baltica in field samples. The probe-based method was tested and optimized in vitro, and potential effects of the B. baltica life-cycle transitions were evaluated by examining the labeling quality of the probes on different life-cycle stages before the assay was applied in a field study. The BbRNA4 probe had the highest specificity and was chosen for downstream applications. The life-cycle experiment showed significant differences in labeling efficiency between cultured cells from different growth phases, particularly for the nutrient-limited treatment compared to the control. Tests with spiked field samples revealed that cell recovery in the FISH assay was low (30%), resulting in a considerable underestimation of in situ abundances. However, a strong relationship between FISH and Utermohl counts of field samples suggests that trends of the bloom dynamics can be followed reliably by this method. By applying the FISH detection method in a field survey, we found that the dynamics of the B. baltica bloom follows that established earlier for the Scrippsiella/Biecheleria/Gymnodinium complex, with 2 peaks throughout the season. When corrected for processing losses, FISH-based abundance estimates suggest that B. baltica comprised the major fraction of the total dinoflagellate complex during the spring bloom at the southwest coast of Finland.

Place, publisher, year, edition, pages
2010. Vol. 61, no 2, 129-140 p.
Keyword [en]
Baltic Sea, Dinoflagellates, FISH, Gulf of Finland, LSU rRNA, Molecular detection, Probes, Spring bloom
National Category
Natural Sciences
URN: urn:nbn:se:su:diva-51448DOI: 10.3354/ame01442ISI: 000284005000003OAI: diva2:385671
authorCount :5Available from: 2011-01-12 Created: 2011-01-10 Last updated: 2011-01-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Sundström, Annica M.Larsson, Ulf
By organisation
Department of Systems Ecology
In the same journal
Aquatic Microbial Ecology
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 16 hits
ReferencesLink to record
Permanent link

Direct link