Testing membrane interactions of CPPs
2011 (English)In: Methods in molecular biology (Clifton, N.J.), ISSN 1940-6029, Vol. 683, 33-40 p.Article in journal (Refereed) Published
The chapter deals with some biophysical methods used for investigating CPP-induced changes in membrane properties by spectroscopy methods such as fluorescence or NMR and methods used for probing CPP-induced leakage in membranes. Some useful model systems for biomembranes are described. These include large unilamellar phospholipid vesicles (LUVs) of well-defined size (diameter typically 100 nm). A protocol for the preparation of such vesicles is included. The leakage studies make use of LUVs with entrapped dye molecules. The NMR studies make use of mixed micelles (bicelles) as a membrane mimetic system, which can be oriented in the magnetic field of the spectrometer.
Place, publisher, year, edition, pages
2011. Vol. 683, 33-40 p.
Fluorescence, NMR, Large unilamellar vesicles, Bicelles, Membrane leakage, Membrane fluidity, Membrane dynamics
Research subject Biophysics; Biochemistry
IdentifiersURN: urn:nbn:se:su:diva-51784DOI: 10.1007/978-1-60761-919-2_3PubMedID: 21053120OAI: oai:DiVA.org:su-51784DiVA: diva2:386035