Change search
ReferencesLink to record
Permanent link

Direct link
Nucleus and Nuclear Envelope: Methods for Preparation
Stockholm University, Faculty of Science, Department of Neurochemistry.
Södertörn University, Stockholm, Sweden.
Stockholm University, Faculty of Science, Department of Neurochemistry.ORCID iD: 0000-0003-2092-457X
2010 (English)Book (Other academic)
Abstract [en]

The cell nucleus of eukaryotic organisms contains the genome surrounded by a nuclear envelope consisting of a double-lipid membrane with embedded nuclear pores and an underlying nuclear lamina. The uniformity in size and density makes it possible to isolate pure intact nuclei at high yields from tissue homogenates by centrifugation through a sucrose cushion. Nuclear envelopes can be prepared from isolated nuclei by enzymatic degradation of their nucleic acid content. The resulting nuclear envelope preparations contain structurally well-conserved inner and outer nuclear membranes with attached ribosomes, nuclear pore complexes and nuclear lamina. Reliable methods for preparation of nuclei and nuclear envelopes play an important role in the successful identification of components that are located in nuclei and in nuclear subcompartments.

Place, publisher, year, edition, pages
Chichester: John Wiley & Sons, Inc. , 2010. , 3 p.1-3 p.
Keyword [en]
nucleus, nuclear envelope, nuclear membrane, nuclear pore
National Category
Neurosciences Cell Biology
URN: urn:nbn:se:su:diva-52742DOI: 10.1002/9780470015902.a0002602.pub2ISBN: Online ISBN: 9780470015902OAI: diva2:388927
RefereegranskatAvailable from: 2011-01-18 Created: 2011-01-18 Last updated: 2015-03-09Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Beckman, MarieHallberg, Einar
By organisation
Department of Neurochemistry
NeurosciencesCell Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 67 hits
ReferencesLink to record
Permanent link

Direct link