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Transient erythromycin resistance phenotype associated with peptidyl-tRNA drop-off on early UGG and GGG codons
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
2007 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 189, no 24, 8993-9000 p.Article in journal (Refereed) Published
Abstract [en]

Expression of minigenes encoding tetra- or pentapeptides MXLX or MXLXV (E peptides), where X is a nonpolar amino acid, renders cells erythromycin resistant whereas expression of minigenes encoding tripeptide MXL does not. By using a 3A' reporter gene system beginning with an E-peptide-encoding sequence, we asked whether the codons UGG and GGG, which are known to promote peptidyl-tRNA drop-off at early positions in mRNA, would result in a phenotype of erythromycin resistance if located after this sequence. We find that UGG or GGG, at either position +4 or +5, without a following stop codon, is associated with an erythromycin resistance phenotype upon gene induction. Our results suggest that, while a stop codon at +4 gives a tripeptide product (MIL) and erythromycin sensitivity, UGG or GGG codons at the same position give a tetrapeptide product (MILW or MILG) and phenotype of erythromycin resistance. Thus, the drop-off event on GGG or UGG codons occurs after incorporation of the corresponding amino acid into the growing peptide chain. Drop-off gives rise to a peptidyl-tRNA where the peptide moiety functionally mimics a minigene peptide product of the type previously associated with erythromycin resistance. Several genes in Escherichia coli fulfill the requirements of high mRNA expression and an E-peptide sequence followed by UGG or GGG at position +4 or +5 and should potentially be able to give an erythromycin resistance phenotype.

Place, publisher, year, edition, pages
2007. Vol. 189, no 24, 8993-9000 p.
Keyword [en]
escherichia-coli k-12, initiation codon, messenger-rna, sequence database, binding domain, ngg codons, amino-acid, protein-a, heterogeneity, dissociation
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-55254DOI: 10.1128/JB.01004-07ISI: 000251992800025OAI: oai:DiVA.org:su-55254DiVA: diva2:402567
Note
authorCount :4Available from: 2011-03-08 Created: 2011-03-07 Last updated: 2017-12-11Bibliographically approved

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