Uptake of cell-penetrating peptides in yeasts
2005 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 579, no 23, 5217-5222 p.Article in journal (Refereed) Published
The uptake of different cell-penetrating peptides (CPPs) in two yeast species, Saccharomyces cerevisiae and Candida albicans, was studied using fluorescence HPLC-analyses of cell content. Comparison of the ability of penetratin, pVEC and (KFF)(3)K to traverse the yeast cell envelope shows that the cellular uptake of the peptides varies widely. Moreover, the intracellular degradation of the CPPs studied varies from complete stability to complete degradation. We show that intracellular degradation into membrane impermeable products can significantly contribute to the fluorescence signal. pVEC displayed highest internalizing capacity, and considering its stability in both yeast species, it is an attractive candidate for further studies.
Place, publisher, year, edition, pages
2005. Vol. 579, no 23, 5217-5222 p.
cell-penetrating peptides, internalization, degradation, Saccharomyces cerevisiae, Candida albicans
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-55654DOI: 10.1016/j.febslet.2005.07.099ISI: 000232194300020OAI: oai:DiVA.org:su-55654DiVA: diva2:405842