Change search
ReferencesLink to record
Permanent link

Direct link
Production and epitope characterization of mAbs specific for translation factor IF1
Stockholm University.
Show others and affiliations
2007 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 364, no 1, 72-78 p.Article in journal (Refereed) Published
Abstract [en]

Initiation of protein synthesis in bacteria relies on the presence of three translation initiation factors, of which translation initiation factor IF I is the smallest having a molecular weight of only 8.2 kDa. In addition to its function in this highly dynamic process, the essential IF1 protein also functions as an RNA chaperone. Despite extensive research, the exact function of IF1 in translation initiation has not yet been determined, and the research in the function of the factor has in some areas been impeded by the lack of monoclonal antibodies specific for this protein. Several attempts to induce immune response in mice with wild-type IF1 for the production of antibodies have failed. We have now succeeded in producing monoclonal antibodies specific for IF1 by applying a new immunization strategy involving an antigen combination of IF1 coupled to glutathione S-transferase (GST) and a recombinant dimer of IF1. This resulted in the generation of 6 IgG 2 IgM, and 1 IgA anti-IF1 antibodies, which can be used in ELISA screening and Western immunoblots. We also provide a mapping of the functional epitopes of the generated anti-IF1 monoclonal antibodies by screening the antibodies for binding to IF1 proteins mutated at single amino acid positions.

Place, publisher, year, edition, pages
2007. Vol. 364, no 1, 72-78 p.
Keyword [en]
monoclonal antibody, translation initiation factor IF1, immunization, glutathione S-transferase, epitope mapping, IF1 mutants
National Category
Natural Sciences
URN: urn:nbn:se:su:diva-55753DOI: 10.1016/j.bbrc.2007.09.120ISI: 000250661500012OAI: diva2:406813
authorCount :5Available from: 2011-03-28 Created: 2011-03-28 Last updated: 2011-03-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Stockholm University
In the same journal
Biochemical and Biophysical Research Communications - BBRC
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 11 hits
ReferencesLink to record
Permanent link

Direct link