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A method for finding sites of selective adenosine deamination
Stockholm University, Faculty of Science, Department of Molecular Biology and Functional Genomics.
Stockholm University, Faculty of Science, Department of Molecular Biology and Functional Genomics.
2007 (English)In: Methods in Enzymology, ISSN 0076-6879, E-ISSN 1557-7988, Vol. 424, 289-300 p.Article, review/survey (Refereed) Published
Abstract [en]

Single sites of selective adenosine (A) to inosine (1) RNA editing with functional consequences on the proteome are rarely found in mammals. Here we describe a method that can be used to detect novel site-selective A-to-I editing in various tissues as well as species. The method utilizes immunoprecipitation of intrinsic RNA-protein complexes to extract substrates subjected to site-selective in vivo editing. We show that known single sites of A-to-I editing are enriched utilizing an antibody against the ADAR2 protein. We propose that this method is suitable for identification of novel substrates subjected to site-selective A-to-I editing.

Place, publisher, year, edition, pages
2007. Vol. 424, 289-300 p.
Keyword [en]
double-stranded-rna, binding domain, messenger-rna, dsrna-binding, editing sites, human brain, adar2, recognition, targets, hairpins
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-55875DOI: 10.1016/S0076-6879(07)24013-3ISI: 000248621500013OAI: oai:DiVA.org:su-55875DiVA: diva2:407442
Note
authorCount :2Available from: 2011-03-30 Created: 2011-03-30 Last updated: 2017-12-11Bibliographically approved

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