A method for finding sites of selective adenosine deamination
2007 (English)In: Methods in Enzymology, ISSN 0076-6879, Vol. 424, 289-300 p.Article, review/survey (Refereed) Published
Single sites of selective adenosine (A) to inosine (1) RNA editing with functional consequences on the proteome are rarely found in mammals. Here we describe a method that can be used to detect novel site-selective A-to-I editing in various tissues as well as species. The method utilizes immunoprecipitation of intrinsic RNA-protein complexes to extract substrates subjected to site-selective in vivo editing. We show that known single sites of A-to-I editing are enriched utilizing an antibody against the ADAR2 protein. We propose that this method is suitable for identification of novel substrates subjected to site-selective A-to-I editing.
Place, publisher, year, edition, pages
2007. Vol. 424, 289-300 p.
double-stranded-rna, binding domain, messenger-rna, dsrna-binding, editing sites, human brain, adar2, recognition, targets, hairpins
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-55875DOI: 10.1016/S0076-6879(07)24013-3ISI: 000248621500013OAI: oai:DiVA.org:su-55875DiVA: diva2:407442
authorCount :22011-03-302011-03-302011-03-30Bibliographically approved