Profilin I and II are both influencing SRF-dependent signaling in B16 melanoma cells and loss of Profilin I interferes with cell migration
(English)Manuscript (preprint) (Other academic)
Profilin is a central component of the microfilament system. Its involvement in controlling actin polymerization associated with transmembrane signaling through its interaction with phosphatidylinositol lipids and a plethora of protein components carrying proline-rich sequence motifs has inspired to a large number of studies. Despite these efforts, however, its function is still not well understood. Therefore we have initiated a study of cultured cells after down-regulation of profilin expression using siRNA. Here we report that depletion of the profilin I and II isoforms in B16 mouse melanoma cells interferes with MAL/SRF-dependent transcription and that loss of profilin reduces their speed of migration as well as their ability to fine-tune their control of the migratory apparatus.
Research subject Cellbiology
IdentifiersURN: urn:nbn:se:su:diva-57369OAI: oai:DiVA.org:su-57369DiVA: diva2:415551