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Cyclobutane pyrimidine dimers do not fully explain the mutagenicity induced by UVA in Chinese hamster cells
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
2008 (English)In: Mutation research, ISSN 0027-5107, E-ISSN 1873-135X, Vol. 648, no 02-jan, 32-39 p.Article in journal (Refereed) Published
Abstract [en]

UVA generates low levels of cyclobutane pyrimidine dimers (CPDs). Here we asked the question whether CPDs could fully explain the level of mutations induced by UVA. Relative mutagenicities of UVA and UVC were calculated at equal levels of CPDs in cell lines, deficient in different aspects of repair. Survival and gene mutations in the hprt locus were analyzed in a set of Chinese hamster ovary (CHO) cell lines, i.e., wild-type, Cockayne syndrome B protein-deficient (CSB), XRCC3-deficient and XRCC1-deficient adjusted to the same level of CPDs which was analyzed as strand breaks as a result of DNA cleavage by T4 endonuclease V at CPD sites. Induced mutagenicity of UVA was approximately 2 times higher than the mutagenicity of UVC in both wild-type and XRCC1-deficient cells when calculated at equal level of CPDs. Since this discrepancy could be explained by the fact that the TT-dimers, induced by UVA, might be more mutagenic than C-containing CPDs induced by UVC, we applied acetophenone, a photosensitizer previously shown to generate enhanced levels of TT-CPDs upon UVB exposure. The results suggested that the TT-CPDs were actually less mutagenic than the C-containing CPDs. We also found that the mutagenic effect of UVA was not significantly enhanced in a cell line deficient in the repair of CPDs. Altogether this suggests that neither base excision- nor nucleotide excision-repair was involved. We further challenge the possibility that the lesion responsible for the mutations induced by UVA was of a more complex nature and which possibly is repaired by homologous recombination (HR). The results indicated that UVA was more recombinogenic than UVC at equal levels of CPDs. We therefore suggest that UVA induces a complex type of lesion, which might be an obstruction during replication fork progression that requires HR repair to be further processed. 

Place, publisher, year, edition, pages
2008. Vol. 648, no 02-jan, 32-39 p.
Keyword [en]
UV, UVA, Mutation, DNA repair, Pyrimidine dimer, Complex damage, replication fork progression, induced dna-damage, homologous recombination, mammalian-cells, wavelength dependence, excision-repair, skin-cancer, bipyrimidine photoproducts, ultraviolet-radiation, molecular-mechanisms
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Natural Sciences
Identifiers
URN: urn:nbn:se:su:diva-57706DOI: 10.1016/j.mrfmmm.2008.09.011ISI: 000261797000005OAI: oai:DiVA.org:su-57706DiVA: diva2:417555
Note
authorCount :4Available from: 2011-05-17 Created: 2011-05-16 Last updated: 2017-12-11Bibliographically approved

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Johansson, FredrikJenssen, DagErixon, Klaus
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