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One SmpB molecule accompanies tmRNA during its passage through the ribosomes
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
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2008 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 582, no 10, 1532-1536 p.Article in journal (Refereed) Published
Abstract [en]

tmRNA and SmpB are the main participants of trans-translation, a process which rescues the ribosome blocked during translation of non-stop mRNA. While a one-to-one stoichiometry of tmRNA to the ribosome is generally accepted, the number of SmpB molecules in the complex is still under question. We have isolated tmRNA-ribosome complexes blocked at different steps of the tmRNA path through the ribosome and analyzed the stoichiometry of the complexes. Ribosome, tmRNA and SmpB were found in equimolar amount in the tmRNA-ribosome complexes stopped at the position of the 2nd, 4th, 5th or the 11th codons of the coding part of the tmRNA. 

Place, publisher, year, edition, pages
2008. Vol. 582, no 10, 1532-1536 p.
Keyword [en]
translation, RNA-protein interaction, trans-translation, tmRNA, SmpB
National Category
Natural Sciences
URN: urn:nbn:se:su:diva-58505DOI: 10.1016/j.febslet.2008.03.049ISI: 000258005200023OAI: diva2:420653
authorCount :5Available from: 2011-06-03 Created: 2011-06-03 Last updated: 2011-06-03Bibliographically approved

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Isaksson, Leif A.
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